Expression and functional SNP loci screen of ATM from coal worker's pneumoconiosis.
10.3760/cma.j.cn121094-20201019-00590
- VernacularTitle:煤工尘肺
ATM基因表达分析及功能性单核苷酸多态性位点筛选
- Author:
Hao DENG
1
;
Tao ZHANG
2
;
Ma Li WU
3
;
Guang Hong YANG
4
;
Yan CHEN
5
;
Yue Dong LIANG
6
Author Information
1. College of Public Health of Guizhou Medical University, Guiyang 550004, China.
2. Occupational Medicine Department of Guiyang Center of Public Health and Treatment, Guiyang 550025, China.
3. Morphological Laboratory of Basic Medical College of Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.
4. Key Laboratory of Environmental Pollution and Disease Monitoring, Ministry of Education, College of Public Health, Guizhou Medical University, Guiyang 550025, China.
5. Institute of Occupational Medicine Prevention, Center of Disease Protection and Control, Guiyang 550004, China.
6. College of Public Health of Guizhou Medical University, Guiyang 550004, China Public Health Treatment Center of Guiyang, Guiyang 550004, China.
- Publication Type:Journal Article
- Keywords:
Ataxia telangiectasia mutated;
Coal workers' pneumoconiosis;
DNA;
DNA damage repair;
Early diagnosis;
Gene;
Pneumoconiosis;
Single nucleotide polymarphism
- MeSH:
Anthracosis/genetics*;
Ataxia Telangiectasia;
Ataxia Telangiectasia Mutated Proteins/genetics*;
China;
Coal;
Coal Mining;
Humans;
Miners;
Pneumoconiosis/epidemiology*;
Polymorphism, Single Nucleotide
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2022;40(2):103-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect of gene expression and genotype of the ataxia telangiectasia mutated (ATM) from coal workers' pneumoconiosis (CWP) , It is explored whether CWP is related to ATM gene. Methods: In October 2020, the relevant information of 264 subjects who received physical examination or medical treatment in the Department of occupational diseases of Guiyang public health treatment center from January 2019 to September 2020 was collected. Through the occupational health examination, 67 healthy people with no history of exposure to occupational hazards were selected as the healthy control group; The coal miners with more than 10 years of coal dust exposure history and small shadow in the lung but not up to the diagnostic criteria were the dust exposure control group, a total of 66 people; The patients with the same history of coal dust exposure and confirmed stage I were coal worker's pneumoconiosis stage I group, a total of 131 people. The expression of ATM was detected by QRT PCR. ATM rs189037 and rs1801516 were genotyped by massarray. Results: There was significant difference in the expression of ATM among the groups (P<0.05) ; Compared with the healthy control group, the expression of ATM in the dust exposed control group was significantly increased (P<0.05) . With the occurrence and development of CWP, the GG of rs189037 wild type decreased, the GA of mutant heterozygote and AA of homozygote increased, but the difference was not statistically significant (P>0.05) ; Rs1801516 wild type GG and mutant heterozygote GA had no significant changes (P>0.05) . There were significant differences in age, neutrophils and basophils among rs189037 groups (all P<0.05) . There were no significant differences in blood pressure, eosinophils, lymphocytes, monocytes, smoking and drinking history among rs189037 groups (all P>0.05) . Compared with wild-type GG, the or of mutant heterozygotes and homozygotes increased, but the differences were not statistically significant (P>0.05) . Conclusion: ATM gene may be one of the early activation genes of CWP and rs189037 may be the functional loci which affects gene expression. ATM gene is related to inflammatory response, Neutrophils and basophils have an impact on the development of CWP.
