Observation of sperm-head vacuoles and sperm morphology under light microscope.
10.5653/cerm.2014.41.3.132
- Author:
Yong Seog PARK
1
;
Sol PARK
;
Duck Sung KO
;
Dong Wook PARK
;
Ju Tae SEO
;
Kwang Moon YANG
Author Information
1. Laboratory of Reproductive Medicine, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine, Seoul, Korea. arkangel@daum.net
- Publication Type:Original Article
- Keywords:
Papanicolaou staining;
Semen analysis;
Sperm-head vacuole;
Sperm morphology
- MeSH:
Age Factors;
Humans;
Male;
Reproductive Techniques, Assisted;
Semen Analysis;
Spermatozoa*;
Vacuoles*;
World Health Organization
- From:Clinical and Experimental Reproductive Medicine
2014;41(3):132-136
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The presence of sperm-head vacuoles has been suspected to be deleterious to the outcomes of assisted reproductive technology (ART). It is difficult to accurately distinguish morphologically abnormal sperm with vacuoles under a light microscope. This study was performed to analyze the result of the observation of sperm-head vacuoles using Papanicolaou staining under a light microscope and whether the male partner's age affects these vacuoles. METHODS: Sperm morphology with vacuoles was evaluated using Papanicolaou staining and observed under a light microscope (400x) in 980 men. The normal morphology was divided into three categories (group A, <4% of normal morphology; group B, 4%-14% of normal morphology; and group C, >14% of normal morphology). The criteria for the sperm-head vacuoles were those given in the World Health Organization manual. For the analysis of the age factor, the participants were divided into the following groups: 26-30 years, 31-35 years, 36-40 years, 41-45 years, and 46-50 years. RESULTS: The percentage of sperm-head vacuoles increased with normal sperm morphology (group A vs. groups B, C) (p<0.05). In the case of the age factor, a statistically significant difference was not observed across any of the age groups. CONCLUSION: A majority of the sperm-head vacuoles showed a statistically significant difference among normal morphology groups. Therefore, we should consider the probability of the percentage of sperm-head vacuoles not increasing with age but with abnormal sperm morphology. A further study is required to clarify the effect of the sperm-head vacuoles on ART outcomes.
