Analysis of HIV-1 genetic subtype and pretreatment drug resistance among men who have sex with men infected with HIV-1 from 19 cities of 6 provinces in China.
10.3760/cma.j.cn112338-20211125-00918
- VernacularTitle:我国6个省19个城市男男性行为人群HIV-1 基因亚型及抗病毒治疗前耐药分析
- Author:
Ran ZHANG
1
;
Ting Li DONG
2
;
Wen Li LIANG
1
;
Zhao Bing CAO
2
;
Zhen XIE
2
;
Kang Mai LIU
2
;
Fei YU
3
;
Geng Feng FU
4
;
Yu Qi ZHANG
5
;
Guo Yong WANG
6
;
Qiao Qin MA
7
;
Shao Bin WU
8
;
Yan LI
9
;
Wei DONG
2
;
Zhen JIANG
2
;
Jie XU
2
;
Zun You WU
2
;
Jun YAO
1
;
Pin Liang PAN
1
;
Mao Feng QIU
1
Author Information
1. National HIV Reference Laboratory, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
2. Division of HIV Prevention and Intervention, National Center for AIDS/STD Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206,China.
3. Danlan Beijing Media Limited, Beijing 100020, China.
4. Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009, China.
5. Hebei Provincial Center for Disease Control and Prevention,Shijiazhuang 050021, China.
6. Shandong Center for Disease Control and Prevention, Ji'nan 250014, China.
7. Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051, China.
8. Fujian Provincial Center for Disease Control and Prevention, Fuzhou 350012,China.
9. Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
- Publication Type:Journal Article
- MeSH:
China/epidemiology*;
Cities;
Drug Resistance;
Drug Resistance, Viral/genetics*;
Female;
Genotype;
HIV Infections/epidemiology*;
HIV Seropositivity/drug therapy*;
HIV-1/genetics*;
Homosexuality, Male;
Humans;
Male;
Phylogeny;
Reverse Transcriptase Inhibitors/therapeutic use*;
Sexual and Gender Minorities
- From:
Chinese Journal of Epidemiology
2022;43(4):523-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the distribution of HIV-1 genetic subtypes and pretreatment drug resistance (PDR) among men who have sex with men (MSM) from 19 cities of 6 provinces in China. Methods: From April to November 2019, 574 plasma samples of ART-naive HIV-1 infected MSM were collected from 19 cities in Hebei, Shandong, Jiangsu, Zhejiang, Fujian, and Guangdong provinces, total ribonucleic acid (RNA) was extracted and amplified the HIV-1 pol gene region by nested polymerase chain reaction (PCR) after reverse transcription. Then sequences were used to construct a phylogenetic tree to determine genetic subtypes and submitted to the Stanford drug resistance database for drug resistance analysis. Results: A total of 479 samples were successfully amplified by PCR. The HIV-1 genetic subtypes included CRF01_AE, CRF07_BC, B, CRF55_01B, CRF59_01B, CRF65_cpx, CRF103_01B, CRF67_01B, CRF68_01B and unrecognized subtype, which accounted for 43.4%, 36.3%, 6.3%, 5.9%, 0.8%, 0.8%, 0.4%, 0.4%, 0.2% and 5.5%, respectively. The distribution of genetic subtypes among provinces is statistically different (χ2=44.141, P<0.001). The overall PDR rate was 4.6% (22/479), the drug resistance rate of non-nucleoside reverse transcriptase inhibitors, nucleoside reverse transcriptase inhibitors, and protease inhibitors were 3.5% (17/479), 0.8% (4/479) and 0.2% (1/479), respectively. The PDR rate of recent infections was significantly higher than that of long-term infections (χ2=4.634, P=0.031). Conclusions: The HIV-1 genetic subtypes among MSM infected with HIV-1 from 19 cities of 6 provinces in China are diverse, and the distribution of subtypes is different among provinces. The overall PDR rate is low, while the PDR rate of recent infections was significantly higher than that of long-term infections, suggesting the surveillance of PDR in recent infections should be strengthened.
