Analysis of hemagglutinin-neuraminidase gene characteristics of human parainfluenza virus type 3 among children with acute respiratory tract infection in Qingdao city.
10.3760/cma.j.cn112150-20211108-01035
- Author:
Kang Yu HAO
1
;
Zi Ran LIU
2
;
Jin Ling GONG
2
;
Rui SUN
2
;
Feng ZHANG
2
;
Wen Jing WANG
1
;
Jia He GAO
3
;
Zhao Guo WANG
4
Author Information
1. Department of Epidemiology and Health Statistics, the College of Public Health of Qingdao University, Qingdao 266071, China.
2. Municipal Centre of Disease Control and Prevention of Qingdao, Qingdao Institute of Prevention Medicine, Qingdao 266033, China.
3. Qingdao Second Middle School in Shandong province, Qingdao 266061, China.
4. Department of Epidemiology and Health Statistics, the College of Public Health of Qingdao University, Qingdao 266071, China Municipal Centre of Disease Control and Prevention of Qingdao, Qingdao Institute of Prevention Medicine, Qingdao 266033, China.
- Publication Type:Journal Article
- MeSH:
Child;
Child, Preschool;
Cross-Sectional Studies;
Female;
Hemagglutinins;
Humans;
Neuraminidase;
Parainfluenza Virus 3, Human/genetics*;
Phylogeny;
Respiratory Tract Infections/epidemiology*;
Viral Proteins
- From:
Chinese Journal of Preventive Medicine
2022;56(5):626-631
- CountryChina
- Language:Chinese
-
Abstract:
The purpose was to discuss the infection status of human parainfluenza virus type 3 (HPIV-3) in children with acute respiratory tract infection(ARTI) in Qingdao, Shandong province, and to analyze the gene characteristics of HPIV-3 hemagglutinin-neuraminidase protein (HN). This study was a cross-sectional study. A total of 1 674 throat swab samples were collected randomly from children with ARTI, in the three hospitals (Qingdao Women and Children's Hospital, West Coast Branch of Affiliated Hospital of Qingdao University, Laoshan Branch of Affiliated Hospital of Qingdao University) from January 2018 to December 2019. Multiplex real-time fluorescence RT-PCR was performed to screen HPIV-3 positive specimens. For HPIV-3 positive specimens, nested PCR was used to amplify the full-length HN gene of HPIV-3. The HN gene was sequenced and compared with the representative strains of HPIV-3 in GenBank, and the phylogenetic tree was established. As results, this study collected 1 674 samples, in which there were 90 HPIV-3 positive samples showed and the detection rate was 5.37%. Among positive specimens, the number of samples from children under 6 years old was 88, accounting for 97.78%. HPIV-3 positive cases were mainly distributed in spring and summer. The full-length sequences of 44 HPIV-3 HN genes were obtained by nested PCR method. Sequence alignment and evolutionary analysis showed that the HPIV-3HN gene belonged to the C3a and C3b branches of C3 genotype, with 30 strains of subtype C3a and 14 strains of subtype C3b. The nucleotide and amino acid homology of the amplified 44 strains of the HPIV-3 HN gene in Qingdao were 97.0%-100.0% and 98.5%-100.0%, respectively. In conclusion, from 2018 to 2019, the C3a and C3b branches of HPIV-3 C3 genotype were circulating prevalent in Qingdao, Shandong province. HN gene variation rate was low, but showed certain regional characteristics in evolution.
