Tracing investigation and analysis of a Clostridium botulinum food poisoning incident in Bayingolin Mongolian Autonomous Prefecture, Xinjiang.
10.3760/cma.j.cn112150-20220118-00066
- VernacularTitle:新疆巴音郭楞蒙古自治州一起肉毒梭菌食物中毒事件的溯源调查分析
- Author:
Yu Jia LIN
1
;
Fang LI
1
;
Jing SU
1
;
Wei Wei MENG
1
;
Tian TIAN
1
;
Yong He YUAN
1
;
Xin MA
1
Author Information
1. Health Monitoring and Testing Center,Center for Disease Prevention and Control of Xinjiang Uygur Autonomous Region,Urumqi 830002,China.
- Publication Type:Journal Article
- MeSH:
Animals;
Clostridium botulinum/genetics*;
Feces;
Foodborne Diseases/epidemiology*;
Gerbillinae;
Humans;
Mice
- From:
Chinese Journal of Preventive Medicine
2022;56(4):541-544
- CountryChina
- Language:Chinese
-
Abstract:
To analyze a suspected case of Clostridium botulinum food poisoning in Bayingolin Mongolian Autonomous Prefecture, Xinjiang and to help validating the diagnosis and providing technical support for clinical treatment. The basic information and clinical manifestations of food poisoning cases were investigated by using the epidemiological method of food safety accidents. The botulinum toxin genes in the samples were detected by real-time PCR and inoculation of KM mouse. The enriched bacteria were further purified and validated. PFGE and cluster analysis were performed on five isolates. Clostridium botulinum type A was detected in two homemade fermented bean samples and stool lavage fluid samples of three patients from enriched samples by toxin test and real-time PCR, and were further validated after isolation of Clostridium botulinum. PFGE showed 100% homology among five isolates. Five isolates of bacteria isolated from the stool lavage fluid of three patients and two homemade fermented bean curd were identified as the same source through PFGE. The cause of this food poisoning cases is food pollution of Clostridium botulinum type A.
