Pathogenic detection and molecular tracing of a bacillary dysentery outbreak by Shigella sonnei in Huainan city.
10.3760/cma.j.cn112150-20210409-00346
- Author:
Jiang LIU
1
;
Yong SUN
2
;
Jie ZHANG
1
;
Fan Rong ZENG
1
;
Xiao Bo WANG
1
;
Li Jie ZHU
1
;
Meng Yang SUN
1
;
Shou Jie YU
1
Author Information
1. Microbiological Laboratory, Huainan City Center for Disease Control and Prevention, Huainan 232033, China.
2. Microbiological laboratory, Anhui Provincial Center for Disease Control and Prevention, Hefei 230601, China.
- Publication Type:Journal Article
- MeSH:
Disease Outbreaks;
Dysentery, Bacillary/microbiology*;
Humans;
Shigella;
Shigella sonnei/genetics*;
Water/pharmacology*
- From:
Chinese Journal of Preventive Medicine
2022;56(4):437-442
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To understand the virulence gene and drug resistance profile of Shigella sonnei outbreak in Huainan city, and conduct pathogenic traceability analysis. Methods: Water samples and feces related to an infectious diarrhea outbreak in Huainan city in August 2020 were collected for multiple pathogen detection. Virulence gene, drug sensitivity, pulse-field gel electrophoresis and whole genome sequencing of Shigella isolates were analyzed respectively. Results: 38 strains of Shigella sonnei were detected in 56 samples of mucilage feces with a positive rate 67.86%, and all serotypes were Shigella sonnei Phase I. Three strains of Shigella sonnei were detected by fluorescence PCR in the Gram-negative (GN) bacterial enrichment solution of terminal water and well water. Virulence genes were ipaH positive (38), ipaH/ial (31) and ipaH/ial/sen positive (1), respectively. The drug resistance spectrum showed that 9 of 14 antibiotics were 100% resistant, and only imipenem, chloramphenicol, ceftazidime and ciprofloxacin were effective drugs. XbaⅠ restriction enzyme map type of 36 isolates was completely consistent, and the ST type analysis of 3 strains was ST152. Whole genome sequencing and analysis verified that the outbreak was caused by a single clonal group of strains, and revealed that the isolates of the outbreak were clustered into a large cluster with 3 Chinese strains and 1 Korean strain in the database, far away from the strains of other countries. Conclusion: The outbreak is caused by a single clone of Shigella sonnei, which are low virulence strains and have multiple drug resistance.
