Identification and analysis of 2 Corynebacterium diphtheria strains in Guangdong Province.
10.3760/cma.j.cn112150-20210517-00478
- Author:
Zhen Cui LI
1
;
Mei Zhen LIU
1
;
Yan Mei FANG
2
;
Zi Jun GONG
3
;
Xu Lin WANG
3
;
Jing Diao CHEN
1
;
Bo Sheng LI
1
Author Information
1. Institute of Pathogenic Microbiology of Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 510000, China Guangdong Workstation for Emerging Infectious Disease Control and Prevention, Chinese Academy of Medical Sciences, Guangzhou 510000, China.
2. Institute of Microbiology of Zhuhai Center for Disease Control and Prenvention,Zhuhai 519000,China.
3. School of Public Health of Guangdong Pharmaceutical University, Guangzhou 510000, China.
- Publication Type:Journal Article
- MeSH:
China/epidemiology*;
Corynebacterium;
Corynebacterium diphtheriae/genetics*;
Diphtheria/microbiology*;
Humans;
Nitrates;
Phylogeny
- From:
Chinese Journal of Preventive Medicine
2022;56(4):427-432
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify and analyze two strains of C. diphtheriae in Guangdong Province by combining whole genome sequencing with traditional detection methods. Methods: The C. diphtheriae was isolated from Guangzhou in 2010 and Zhuhai in 2020 respectively. Isolates were identified by API Coryne strips and MALDI-TOF-MS. Genomic DNA was sequenced by using Illumina. The assembly was performed for each strain using CLC software. J Species WS online tool was used for average nucleoside homology identification, then narKGHIJ and tox gene were detected by NCBI online analysis tool BLSATN. MEGA-X was used to build a wgSNP phylogenetic tree. Results: GD-Guangzhou-2010 was Belfanti and GD-Zuhai-2020 was Gravis. ANIb between GD-Guangzhou-2010 and C. belfantii was 99.61%. ANI between GD-Zhuhai-2020 and C. diphtheriae was 97.64%. BLASTN results showed that the nitrate reduction gene narKGHIJ and tox gene of GD-Guangzhou-2010 was negative, while GD-Zhuhai-2020 nitrate reduction gene narKGHIJ was positive. There were two obvious clades in wgSNP phylogenetic tree. The first clades included all Mitis and Gravis types strains as well as GD-Zhuhai-2020. The second clades contained all isolates of C.belfantii, C.diphtheriae subsp. lausannense and GD-guangzhou-2010. Conclusion: Two non-toxic C. diphtheriae strains are successfully isolated and identified. The phylogenetic tree suggests that GD-Guangzhou-2010 and GD-Zhuhai-2020 are located in two different evolutionary branches.
