Perifosine inhibits biofilm formation of Pseudomonas aeruginosa by interacting with PqsE protein.
10.3760/cma.j.cn112150-20211020-00970
- VernacularTitle:哌立福新通过结合PqsE蛋白抑制铜绿假单胞菌生物膜的形成
- Author:
Peng Fei SHE
1
;
Lan Lan XU
1
;
Ya Qian LIU
1
;
Ze Hao LI
1
;
Sha Sha LIU
1
;
Yi Min LI
1
;
Lin Ying ZHOU
2
;
Yong WU
2
Author Information
1. Department of Laboratory Medicine, Third Xiangya Hospital, Central South University, Changsha 410013, China.
2. Department of Laboratory Medicine, the First Hospital of Changsha, Changsha 410005, China.
- Publication Type:Journal Article
- MeSH:
Anti-Bacterial Agents/pharmacology*;
Bacterial Proteins/metabolism*;
Biofilms;
Molecular Docking Simulation;
Phosphorylcholine/analogs & derivatives*;
Pseudomonas aeruginosa/metabolism*;
Quorum Sensing
- From:
Chinese Journal of Preventive Medicine
2022;56(2):192-196
- CountryChina
- Language:Chinese
-
Abstract:
To explore the biofilm inhibitory efficacy of perifosine against Pseudomonas aeruginosa (P. aeruginos) and its mechanisms. Twenty-fourwell plate was used to form biofilms at the bottom and crystal violet staining was used to determine the biofilm inhibitory effects of perifosine against P. aeruginosa, the wells without perifosine was set as control group. Glass tubes combined with crystal violet staining was used to detect the gas-liqud interface related bioiflm inhibitory effects of perifosine, the wells without perifosine was set as control group. Time-growth curved was used to detect the effects of perifosine on the bacteial planktonic cells growth of P. aeruginosa, the wells without perifosine was set as control group. The interaction model between perifosine and PqsE was assessed by molecular docking assay. The inhibitory effects of perifosine on the catalytic activity of PqsE was determined by detection the production of thiols, the wells without perifosine was set as control group. Binding affinity between perifosine and PqsE was detected by plasma surface resonance. The biofims at the bottom of the microplates and air-liquid interface were effectively inhibited by perifosine at the concentration of 4-8 μg/ml. There was no influence of perifosine on the cells growth of P. aeruginosa. The resuts of molecular docking assay indicates that perifosine could interacted with PqsE with the docking score of -10.67 kcal/mol. Perifosine could inhibit the catalytic activity of PqsE in a dose-dependent manner. The binding affinity between perifosine and PqsE was comfirmed by plasma surface resonance with KD of 6.65×10-5mol/L. Perifosine could inhibited the biofilm formation of P. aeruginosa by interacting with PqsE.
