The in vivo imaging of zebrafish for chemical vascular toxicity assessment.
10.3760/cma.j.cn112150-20210510-00457
- Author:
Di JI
1
;
Qi Cheng ZHU
1
;
Yan Hong WEI
1
Author Information
1. Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Genetically Modified;
Cardiovascular System;
Toxicity Tests;
Zebrafish
- From:
Chinese Journal of Preventive Medicine
2022;56(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the vascular toxicity of chemicals by a real-time observation approach using the transgenic zebrafish. Methods: The spatiotemporal vascular alterations of transgenic zebrafish after chemical exposure were assessed by laser confocal microscopy and high-content screening analysis, respectively. Results: The method using Laser Confocal Microscopy (LCM) is easier to operate and yields high-resolution images, while it is lower throughput and inefficient. In contrast, high-content analysis (HCA) analysis obtains high-quality data of vascular toxicity manifesting whole blood vasculature, whereas it requires delicate operation procedures and advanced experimental conditions. Conclusion: Two kinds of zebrafish imaging methods each have advantages and disadvantages. LCM is suitable for the evaluation of a small number of chemicals. HCA, a cutting-edge technology, has great potential for chemical safety assessment allowing high throughput vascular toxicity tests of a good number of chemicals at a time.
