Application of single-cell transcriptome sequencing in mechanistic toxicology.
10.3760/cma.j.cn112150-20210513-00469
- VernacularTitle:单细胞转录组测序在毒理学机制研究中的应用
- Author:
Yue Jin YU
1
;
Zhu Yi ZHANG
1
;
Yan Hong WEI
1
Author Information
1. Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
- Publication Type:Review
- MeSH:
Base Sequence;
Gene Expression Profiling;
Sequence Analysis, RNA;
Single-Cell Analysis;
Transcriptome
- From:
Chinese Journal of Preventive Medicine
2022;56(1):29-32
- CountryChina
- Language:Chinese
-
Abstract:
Traditional bulk RNA sequencing assesses the average expression level of genes in tissues rather than the differences in cellular responses. Accordingly, it is hard to differentiate sensitive responding cells, leading to inaccurate identification of toxicity pathways. Single-cell RNA sequencing (scRNA-seq) isolated single cells from tissue and subjected them to cell subtypes-specific transcriptome analysis. This technique in toxicological studies realizes the heterogeneous cellular responses in the tissue microenvironment upon chemical exposure. Thus it helps to identify sensitive responding cells and key molecular events, providing a powerful tool and a new perspective for exploring the mechanisms of toxicity and the modes of action. This review summarizes the development, principle, method, application and limitations of scRNA-seq in mechanistic toxicological researches, and discusses the prospect of multi-directional applications.
