Progress in the application of three-dimensional cell culture model in toxicity tests of xenobiotic.
10.3760/cma.j.cn112150-20210507-00446
- VernacularTitle:3D细胞模型在化学物质毒性测试中的应用研究进展
- Author:
Zi Wei WANG
1
;
Rui ZHANG
1
;
Wen CHEN
1
Author Information
1. Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.
- Publication Type:Review
- MeSH:
Cell Culture Techniques;
Cell Culture Techniques, Three Dimensional;
Cell Line;
Humans;
Toxicity Tests;
Xenobiotics/toxicity*
- From:
Chinese Journal of Preventive Medicine
2022;56(1):20-24
- CountryChina
- Language:Chinese
-
Abstract:
In the process of xenobiotic toxicity prediction and risk assessment, in vitro cell culture models possess high practical application value. With the rapid development of biological technologies such as three-dimensional (3D) bio-printing, organoid culture and organ-on-a-chip systems, in vitro cell culture models have made great progress. Sharing the similarities in structure, function and the physiological environment with tissues or organs in vivo, hazard identification and dose-response analysis based on 3D cell culture models provide access to more accurate toxicity data as a theoretical basis for risk assessment and risk management of chemicals. This review summarizes the establishment of three typical 3D cell culture models, i.e., human cell line-based co-culture model, 3D-printed scaffold-based cell culture model and organoids, and their application in toxicity tests of xenobiotics.
