Rapid analysis of chemical components of Xiaotan Tongfu formula based on UHPLC-Q-TOF/MS
10.12206/j.issn.1006-0111.202109029
- VernacularTitle:基于UHPLC-Q-TOF/MS技术快速分析消痰通腑方化学成分
- Author:
Ye QIN
1
;
Wei HUANG
2
;
Yufang GU
3
;
Ci’an ZHANG
3
;
Lijuan XIU
3
;
Jigui TANG
3
;
Feng ZHANG
3
;
Xuan LIU
3
Author Information
1. the General Hospital of the Central Theater Command of the PLA, Wuhan 430014, China.
2. Gumei Community Health Service Center, Shanghai 201102, China.
3. Changzheng hospital, Naval Medical University, Shanghai 200003, China.
- Keywords:
UHPLC-Q-TOF/MS;
chemical components;
Xiaotan Tongfu formula
- From:
Journal of Pharmaceutical Practice
2022;40(4):339-346
- CountryChina
- Language:Chinese
-
Abstract:
Objective To rapidly explore the chemical components of Xiaotan Tongfu formula, and to provide scientific basis for the basic research and clinical treatment of the formula. Methods Analysis was performed on an Agilent 1290 ultra-performance liquid chromatography system coupled with an Agilent 6530 accurate quality Q-TOF/MS system, by using a Waters ACQUITY UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm), with a gradient elution applying 0.1% aqueous formic acid solution and acetonitrile as a mobile phase. The flow rate was 0.3 ml/min. The column temperature was 30°C. The injection volume was 1 μl, and the detection wavelength was 254 nm. Mass spectrometry (MS) data were collected in both positive and negative ESI ion modes. Components in the formula were identified by using the in-house compound database, and comparing the retention time (tR), MS1 and MS2 data with the standard compounds, and the online compound MS database. Results A total of 55 compounds were identified from Coptis coptidis, Pseudomonas solani, Rhubarb, Araceae artemisiae and Pinellia chinensis. Conclusion The established UHPLC-Q-TOF/MS method could systematically and accurately identify the chemical components from Xiaotan Tongfu formula, and provided a reference for the quality marker selection and the research on the active ingredient.
