Effect and mechanism of estrogen on EPCs function in diabetic rats
10.12206/j.issn.1006-0111.202106121
- VernacularTitle:雌激素对糖尿病大鼠内皮祖细胞功能的影响及其机制研究
- Author:
Yafen DONG
1
;
Jian WANG
1
;
Li LI
1
;
Shushu LI
1
;
Helong LIU
1
;
Yan QIU
1
Author Information
1. Shanghai Pudong New Area People’s Hospital, Shanghai 201200, China.
- Keywords:
estrogen;
endothelial progenitor cells;
diabetes;
TSP-1;
MnSOD;
NO
- From:
Journal of Pharmaceutical Practice
2022;40(4):320-325
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and mechanism of estrogen on endothelial progenitor cells（EPCs）function in diabetic rats. Methods EPCs were isolated from bone marrow of rats and characterized by fluorescence microscopy and flow cytometry. Rat diabetic model was established via streptozotocin induction. The bone marrow was taken to culture EPCs. EPCs of diabetes were incubated with Estrogen 10 nmol/L for 24h. The functions and proliferation of EPCs in vitro were detected. The levels of MnSOD and NO in EPCs and TSP-1 in supernatant were assayed. Results Compared with control group, EPCs proliferation, adhesion and angiogenesis functions were impaired in diabetic rats. The level of MnSOD and NO in diabetic EPCs were significantly decreased, while TSP-1 protein level in the supernatant increased. The above changes can be reversed with estrogen incubation. Conclusion Estrogen improved the EPCs migration and tubule formation in diabetic rats. The mechanism may be related to the reduction of oxidative stress and downregulation of TSP-1 expression in diabetic EPCs.
