Protective effects of the total bakkenolides from Petasites tricholobus on hypoxia mice under normobaric pressure
10.12206/j.issn.1006-0111.202111085
- VernacularTitle:蜂斗菜总内酯对常压缺氧小鼠的保护作用研究
- Author:
Bingfeng LI
1
;
Yaqian DUAN
1
;
Xu WANG
2
;
Meili GUO
1
;
Yue GAO
3
,
4
Author Information
1. 1a.School of Pharmacy, 1b. Clinical Research Unit of the First Affiliated Hospital, Naval Medical University, Shanghai 200433, China.
2. Military Hospital of No. 63600 troops of the PLA, Jiuquan 732750, China.
3. 1a.School of Pharmacy, 1b. Clinical Research Unit of the First Affiliated Hospital, Naval Medical University, Shanghai 200433, China
4. Clinical Research Unit of the First Affiliated Hospital, Naval Medical University, Shanghai 200433, China.
- Keywords:
Petasites.tricholobus;
anti-hypoxia;
oxygen free radicals;
pathological dyeing;
cell experiment
- From:
Journal of Pharmaceutical Practice
2022;40(4):314-319
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of the total bakkenolides from P.tricholobus on improving hypoxia tolerance in mice. Methods Mice normobaric pressure hypoxia model and oxygen glucose deprivation model in PC12 cells were established, and the effects of PTB on survival time, serum lactate dehydrogenase (LDH) activity and malondialdehyde (MDA) content, brain and heart superoxide dismutase (SOD) and reduced glutathione (GSH) activities, brain tissue pathological changes and cell survival were observed. Results The total bakkenolides from P.tricholobus had prolonged the survival time of mice in confined spaces, increased the activity of SOD and GSH, reduced the production of lipid peroxidation, decreased the degree of anaerobic glycolysis, protected the structure and function of neural cells, and improved the survival rate of OGD-treated cells. Conclusion The total bakkenolides from P.tricholobus could promote the hypoxia tolerance in mice which might be related to scavenging oxygen free radicals, inhibiting lipid peroxidation reaction and protecting the structures and functions of nerve cells.
