Optimization of the extraction technology of the leaves of Dimocarpus longan by Box-Behnken response surface methodology combined with multi-index comprehensive score
- VernacularTitle:Box-Behnken响应面法结合多指标综合评分法优化龙眼叶提取工艺
- Author:
Guangqiang HUANG
1
;
Piaoxue ZHENG
1
;
Jie LIANG
1
,
2
,
3
,
4
,
5
;
Kuikui CHEN
1
;
Yupin CAO
1
;
Jue HU
1
;
Shijia AN
1
;
Jingchun LIANG
1
;
Xingchen LIU
1
;
Xiaofeng ZHU
5
Author Information
1. School of Pharmacy，Guangxi University of TCM，Nanning 530200，China
2. Guangxi Zhuang Yao Medicine Center of Engineering and Technology，Nanning 530200，China
3. Guangxi Superior Chinese Patent Medicine and Ethnic Medicine Development Engineering Technology Center，Nanning 530200，China
4. South China Branch of National Engineering Research Center for Manufacturing Technology of Traditional Chinese Medicine Solid Preparation，Nanning 530200，China
5. School of TCM，Jinan University，Guangzhou 510632，China
- Publication Type:Journal Article
- Keywords:
leaves of Dimocarpus longan;
Box-Behnken
- From:
China Pharmacy
2022;33(14):1688-1693
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTI VE To optimize the extraction technology of the leaves of Dimocarpus longan according to flavonoids and phenolic acids. METHODS The contents of gallic acid ，protocatechuic acid ，ethyl gallate ，quercetin，luteolin and kaempferol in the leaves of D. longan were determined by HPLC. Based on single factor test ，with the ethanol volume fraction ，solid-liquid ratio and extraction time as factors ，using comprehensive scores of the contents of above six components as indexes ，the extraction technology of the leaves of D. longan was optimized by Box-Behnken response surface methodology. RESULTS The optimal extraction technology included ethanol volume fraction of 100％，solid-liquid ratio of l ∶ 7（g/mL），extraction time of 90 min， extraction temperature of 80 ℃. After 3 times of validation tests ，the average comprehensive score was 97.54（RSD＝0.33％，n＝ 3），relative error of which with predicted score （99.05）was 1.55％. CONCLUSIONS Box-Behnken response surface methodology combined with multi-index comprehensive score can be used for the extraction technology of the leaves of D. longan ，and the optimized extraction technology is stable and feasible.
