Effect of Electroacupuncture on Learning and Memory Ability of Cerebral Ischemia/Reperfusion Injured Rats
10.3969/j.issn.1006-9771.2014.09.010
- VernacularTitle:电针对局灶性脑缺血再灌注损伤大鼠学习记忆功能的影响
- Author:
Yukun LIN
;
Jing TAO
;
Bin CHEN
;
Ruhui LIN
- Publication Type:Journal Article
- Keywords:
cerebral ischemia/reperfusion, electroacupuncture, learning and memory, apoptosis, Bax, Bcl-2
- From:
Chinese Journal of Rehabilitation Theory and Practice
2014;(9):831-834
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of electroacupuncture at Shenting (DU24) and Baihui (DU20) on learning and memory ability, as well as the nerve cell and the expression of mRNA of Bcl-2 and Bax in the hippocampus of rats with cerebral ischemia/reperfusion (I/R) injury. Methods 45 male Sprague-Dawley rats were randomly diveded into sham group (n=15), control group (n=15) and electroacupuncture group (n=15). The latter 2 groups were modeled with middle cerebral artery occlusion for 2 h and reperfusion. The electroacupuncture group received electroacupuncture at Shenting and Baihui for 7 days. They were tested with Morris water maze, and then, their hippocampus was observed under Nissl staining and the level of Bcl-2 and Bax mRNA were determined with RT-PCR. Results The escape latency decreased (P<0.001) and the frequence the platform was crossed increased (P=0.001) in the electroacupuncture group compared with the model group, with less damage of the nerve cell and the expression of Bax mRNA, and more of Bcl-2 mRNA (P<0.05). Conclusion Electroacupuncture can prevent learning and memory ability from cerebral ischemia/reperfusion injury in rats, which may associate with adjustment of expression of apoptosis-related gene to inhibit apoptosis of nerve cell.
