Research progress and application of donor-derived cell-free DNA in diagnosis and treatment of kidney transplantation
10.3969/j.issn.1674-7445.2022.04.007
- VernacularTitle:供者来源性细胞游离DNA在肾移植诊疗中的研究进展与应用
- Author:
Yang YANG
1
;
Jian ZHANG
;
Jun LIN
Author Information
1. Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
- Publication Type:Research Article
- Keywords:
Kidney transplantation;
Donor-derived cell-free DNA (dd-cfDNA);
Rejection;
Biomarker;
Allograft injury;
Antibody-mediated rejection (AMR);
T-cell mediated rejection (TCMR);
BK virus-associated nephropathy (BKVAN)
- From:
Organ Transplantation
2022;13(4):455-
- CountryChina
- Language:Chinese
-
Abstract:
Early diagnosis and treatment of rejection after kidney transplantation play a critical role in alleviating allograft injury. Detection of donor-derived cell-free DNA (dd-cfDNA) could be performed based on the next-generation sequencing and other techniques. The content of DNA fragments derived from necrotic and apoptotic donor kidney tissues in circulating body fluids could be determined by concentration and absolute quantitative methods, which has application potential in monitoring allograft injury in clinical practice. Compared with traditional serum creatinine and other indicators, dd-cfDNA detection may monitor allograft injury from several weeks to months in advance, providing a "time window" for clinical treatment and delaying graft failure. Along with deepening research of dd-cfDNA in recent years, dd-cfDNA has captivated widespread attention due to its non-invasiveness, high sensitivity and real-time evaluation of therapeutic effect. In this article, current study evidence and conclusions related to multidimensional application of dd-cfDNA detection in diagnosis and treatment of kidney transplantation were reviewed, and the future research and clinical application direction of dd-cfDNA were discussed, aiming to provide reference for widespread application of dd-cfDNA detection in clinical practice in China.
