Depression of Both Interferon-gamma and Tumor Necrosis Factor-alpha Production by Peripheral Blood Mononuclear Cells from Chronic Refractory Tuberculosis Patients.
- Author:
Ji Sook LEE
1
;
So Hyun LEE
;
Chang Hwa SONG
;
Jae Hyun LIM
;
Hwa Jung KIM
;
Jeong Kyu PARK
;
Tae Hyun PAIK
;
Chul Hee KIM
;
Suck Jun KONG
;
Mal Hyun SHON
;
Eun Kyeong JO
Author Information
1. Department of Microbiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-ku, Daejeon, Korea. hayoungj@cnu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Mycobacterium tuberculosis;
Interferon-gamma;
Interleukin-12;
Interleukin-10;
Multidrug-resistant tuberculosis
- MeSH:
Depression*;
Humans;
Immunotherapy;
Interferon-gamma*;
Interleukin-10;
Interleukin-12;
Interleukin-6;
Interleukins;
Mycobacterium tuberculosis;
Tuberculin;
Tuberculosis*;
Tuberculosis, Multidrug-Resistant;
Tumor Necrosis Factor-alpha*
- From:Journal of Bacteriology and Virology
2002;32(4):393-400
- CountryRepublic of Korea
- Language:English
-
Abstract:
Understanding human immune responses in chronic refractory tuberculosis (CRTB) is important for developing immunotherapy against the disease. The aim of this study was to examine cytokine responses [interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-10] by peripheral blood mononuclear cells (PBMCs) in CRTB patients after in vitro stimulation with the 30-kDa or purified protein derivative (PPD) antigen (Ag). Most of the CRTB cases were multidrug-resistant (MDR) TB. The results were compared with those from early TB (E-TB) patients and healthy tuberculin reactors (HTR). IFN-gamma production was significantly depressed in both CRTB and E-TB groups compared with HTR. In response to the 30-kDa Ag, TNF-alpha levels were significantly depressed only in CRTB patients, while greatly increased in E-TB patients. In addition, IL-10 production was significantly increased in E-TB patients, and PBMC from both E-TB and CRTB patients secreted more IL-6 than HTR. IL-10 neutralization significantly increased TNF-alpha levels, whereas anti-TNF-alpha did not alter IL-10 induction significantly in PBMC from HTR and CRTB patients. Our findings suggest that CRTB patients have depression in both IFN-gamma and TNF-alpha reponses, which might play important roles during chronic M. tuberculosis infection.
