Effects of Cluster Scalp Acupuncture Combined with Enriched Environment Stimulationon Proliferationand Migration of Endogenous Neural Stem Cells in Neonatal Rats with Hypoxic-ischemic Brain Damage
10.3969/j.issn.1006-9771.2014.06.007
- VernacularTitle:头穴丛刺结合丰富环境刺激对缺氧缺血性脑损伤新生大鼠内源性神经干细胞增殖迁移的影响
- Author:
Bo LIU
1
;
Wei ZOU
1
;
Qiang TANG
1
;
Peiyu YAN
1
Author Information
1. TheSecondAf-filiatedHospitalofHeilongjiangUniversityofChineseMedicine,Harbin150001,Heilongjiang,China
- Publication Type:Journal Article
- Keywords:
hypoxic-ischemicbraindamage, clusterscalpacupuncture, enrichedenvironmentalstimulation, proliferation, migration, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2014;(6):533-536
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveToexploretheeffectofclusterscalpacupuncturecombinedwithenrichedenvironmentalstimulationonprolifera-tionandmigrationofendogenousneuralstemcells(NSCs)inneonatalratswithhypoxic-ischemicbraindamage(HIBD).MethodsHIBD modelwasestablishedwithRicemethod.120ratswererandomlydividedintoshamoperationgroup(A),modelgroup(B),clusterscalpacu-puncturegroup(C),enrichedenvironmentalstimulationgroup(D)andclusterscalpacupuncturecombinedwithenrichedenvironmental stimulationgroup(E)with24ratsineachgroup,eachgroupwasdividedinto3days,7daysand14dayssubgroups.Immunohistochemical methodwasusedtoobservethepositivecellsofBromodeoxyuridine(BrdU)andnestin.ResultsComparedwithGroupB,thepositivecells numberofBrdUandnestininsubventricularzone(SVZ)andcerebralcortexincreasedinGroupsCandDatallthetimepoints(P<0.01), andthenumberwasmoreinGroupEthaninGroupsCandD(P<0.05).ConclusionClusterscalpacupuncturecombinedwithenricheden-vironmentalstimulationcouldpromotetheproliferationandmigrationofendogenousNSCsinSVZandcerebralcortexinneonatalratswith HIBD.
