EstablishmentofAnimalModelofEarlyOsteonecrosisoftheFemoralHeadwithModified-inducedSteroidMethod
10.3969/j.issn.1006-9771.2014.06.006
- VernacularTitle:改良激素法建立早期股骨头坏死动物模型
- Author:
Hongwei MIN
1
,
2
;
Kemin LIU
1
,
2
;
Anqing WANG
1
,
2
;
Xinzha HAN
1
,
2
;
Rui GU
1
,
2
Author Information
1. CapitalMedicalUniversitySchoolofRehabilitationMedicine,DepartmentofOrthopedics,BeijingBo'
2. ai Hospital,ChinaRehabilitationResearchCenter,Beijing100068,China
- Publication Type:Journal Article
- Keywords:
osteonecrosisofthefemoralhead, earlystage, modifiedsteroid-inducedmethod, animalmodel, Beagles
- From:
Chinese Journal of Rehabilitation Theory and Practice
2014;(6):527-532
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveToexplorethemethodtoestablishanimalmodelofearlyosteonecrosisofthefemoralhead(ONFH)inducedby steroid.Methods20healthymaleBeagleswererandomlydividedintocontrolgroupandexperimentalgroupwith10dogsineachgroup. Theexperimentalgroupwasinjectedlipopolysaccharide10μg/kgandmethylprednisolone20mg/kgfor3daysconsecutively.Thecontrol groupwasinjectednormalsaline.2monthsand4monthsafteradministration,bothgroupswereperformedmagneticresonanceimaging (MRI).5animalsweresacrificedrespectivelyat2monthsand4monthsafteradministrationineachgroup,andbilateralfemoralheadspeci-menswereobtainedtoperformhistologicalexamination.Plasmaprothrombintime(PT),activatedpartialthromboplastintime(APTT),anti-thrombinIII(AT-III)weretestedbeforeand24hafteradministration.ResultsIntheexperimentalgroup,thepathologicalresultsshowed thattherewere4ONFH2monthsand6ONFH4monthsafteradministrationandMRIdidnotshowanyabnormality.Comparedwiththe controlgroup,thePT,APTT,AT-IIIintheexperimentgroupshortenedsignificantlyafteradministration(P<0.001).ConclusionModified steroid-inducedmethodcanestablishtheanimalmodelofearlyONFH.Hypercoagulationandlowfibrinolysismaybethereasonofste-roid-inducedosteonecrosis.
