Expression and Characterization of Human Immunodeficiency Virus Type 1 Envelope Mutant Glycoproteins by Using Baculovirus Expression System.
- Author:
Yoon LEE
1
;
Ji Yoon RYU
;
Kil Soo LEE
;
Yong Soo BAE
;
Soo Young CHOI
;
Jin Seu PARK
Author Information
1. Division of Biological Sciences, College of Natural Science, Hallym University, Chunchon, Korea. jinpark@hallym.ac.kr
- Publication Type:Original Article
- Keywords:
HIV;
Envelope glycoprotein;
Baculovirus;
Expression;
Secretion
- MeSH:
Baculoviridae*;
Glycoproteins*;
HIV*;
HIV-1*;
Humans*;
Insects;
Membranes;
Staphylococcal Protein A;
Virion
- From:Journal of Bacteriology and Virology
2002;32(4):431-440
- CountryRepublic of Korea
- Language:English
-
Abstract:
Considerable effort has been directed at understanding the structure and function of HIV-1 envelope glycoproteins. It has been difficult to characterize HIV-1 envelope glycoproteins due to the limited availability of these proteins from virus particles or infected cells. To facilitate the structural and functional analysis of HIV-1 envelope glycoproteins, recombinant baculoviruses were generated to express wild type or mutant HIV-1 envelope glycoproteins. The gp160 precursor protein as well as the gp120 glycoprotein were detected in the cell infected with recombinant BacENVw.t containing wild type HIV-1 envelope gene. In the insect cells infected with recombinant BacENVc with mutations at the cleavage site of gp160, a precursor form of envelope glycoprotein was produced, but not secreted into the culture medium. However, the insect cells infected with recombinant BacENVc/t containing both mutations at the cleavage site and membrane spanning region produced mutant envelope glycoproteins that were efficiently secreted into the culture medium in the form of precursor. Therefore the recombinant HIV-1 envelope glycoproteins produced in this system would be useful as immunogens in the development of a vaccine against AIDS.
