Effects of scutellaria on the proliferation and migration of cervical cancer cells through miRNA-496 and stromal cell-derived factor 1
10.3760/cma.j.cn115355-20210708-00300
- VernacularTitle:野黄芩素通过miRNA-496和基质细胞衍生因子1对子宫颈癌细胞增殖及迁移的影响
- Author:
Liulian ZHANG
1
;
Mengxue WU
;
Qianqian WANG
;
Pan LI
;
Lingmeng ZHAO
Author Information
1. 华中科技大学同济医学院附属武汉中心医院妇产科，武汉 430014
- Keywords:
Uterine cervical neoplasms;
MicroRNAs;
Scutellarin;
Cell proliferation;
Cell migration
- From:
Cancer Research and Clinic
2022;34(3):161-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of scutellarein on the cell proliferation and migration of cervical cancer cell line HCC94 through miRNA-496 (miR-496) and stromal cell-derived factor 1 (SDF-1).Methods:HCC94 cells in the logarithmic growth phase were taken as objects, and 20 μmol/L scutellarin solution (scutellarin group) and dimethyl sulfoxide (DMSO) (control group) were added respectively. The CCK-8 method and Transwell experiment were used to detect the proliferation and migration ability of the two groups of HCC94 cells. The relative expression levels of miR-496 and SDF-1 mRNA were detected by real-time quantitative polymerase chain reaction (qRT-PCR). The target gene of miR-496 was predicted by the bioinformatics software TarBase and verified by the dual-luciferase reporter gene assay. Western blot was used to detect the expression of related proteins.Results:Compared with the control group, the proliferation ability of HCC94 cells in the scutellarin group was decreased on the 3rd, 4th and 5th day of culture (all P < 0.05). The number of HCC94 cells in the scutellarin group and the control group were 25±5 and 134±19, respectively, and the cell migration ability of the scutellarin group was lower than that of the control group ( t = 5.61, P < 0.01). The relative expressions of miR-496 in the control group and the scutellarin group were 1.07±0.12 and 11.24±2.75, respectively, and the difference was statistically significant ( t = 3.68, P < 0.01). The dual-luciferase reporter gene assay confirmed that SDF-1 was the downstream targeted gene of miR-496. The relative expressions of SDF-1 mRNA in the scutellarin group and the control group were 0.29±0.05 and 1.01±0.07, respectively, and the difference was statistically significant ( t = 7.22, P < 0.01). Compared with the control group, after scutellarin promoted the expression of miR-496, the expressions of SDF-1 protein, the cell proliferation protein cyclin-dependent kinase 3 (CDK3) and the cell migration proteins Slug and Zeb-2 were decreased. Conclusions:Scutellarin could inhibit the proliferation and migration of cervical cancer HCC94 cells through the miR-496-SDF-1 axis.
