A study on keloid fibroblast induced THP-1 derived macrophage polarization and inflammatory factor TNF-αexpression and its feedback effect
10.3760/cma.j.issn.1671-0290.2022.02.015
- VernacularTitle:瘢痕疙瘩成纤维细胞诱导人急性单核细胞白血病细胞来源巨噬细胞极化与肿瘤坏死因子-α表达的研究
- Author:
Hua CHAO
1
;
Zhicheng SU
;
Ruixi ZENG
;
Borui XU
;
Qing TANG
Author Information
1. 中山大学附属第一医院整形外科，广州 510000
- Keywords:
Keloid;
Co-culture;
Primary fibroblast;
Macrophage;
Tumour necrosis factor-α
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2022;28(2):130-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of keloid fibroblasts on the polarization and expression of inflammatory factors of M0 macrophages and possible mechanisms, and provide theoretical basis for new targets for keloid therapy.Methods:Keloids, normal skin tissues and paraffin specimens from patients undergoing plastic surgery in the First Affiliated Hospital of Sun Yat-sen University from November 2020 to September 2021 were collected, and fibroblasts of keloids and normal skins were isolated and co-cultured with M0 cells formed form THP-1 by phorbol ester (PMA)-stimulation to detect the expression of macrophage polarization markers and cytokines. Besides, keloid fibroblasts were treated with exogenous tumor necrosis factor-α(TNF-α) to detect its effect on the proliferation and extracellular matrix expression.Results:Macrophages were dominated by CD163 + (M2) in keloid tissues. Moreover, M0 cells expressed more TNF-α when co-cultured with keloid fibroblasts, compared with those with normal skin fibroblasts, in which, the positive staining rates of TNF-α were 19.32% and 29.52% respectively by flow cytometry. Furthermore, the proliferation was promoted and the expression of extracellular matrix proteins (COL3A1 and FN1)and Vimentin were upregulated in keloid fibroblasts under TNF-α stimulation. However, there was no significant difference in the expression of polarization surface markers CD86 and CD163 in macrophages, when co-cultured with keloid fibroblasts or normal skin fibroblasts. Conclusions:Keloid fibroblasts promote the expression of TNF-α in macrophages, which in turn promotes the proliferation and extracellular matrix secretion of keloid fibroblasts.
