Use of modified gelatin/PCL electrospun membranes in engineering bilayered skin graft
10.3760/cma.j.issn.1671-0290.2022.01.016
- VernacularTitle:改良明胶/聚己内酯复合纳米纤维电纺膜构建复层皮肤的研究
- Author:
Junxian LI
1
;
Yehong ZHONG
;
Liang XU
;
Zheyuan YU
;
Huichuan DUAN
;
Jie YUAN
Author Information
1. 山西省人民医院口腔颌面外科，太原 030002
- Keywords:
Tissue engineering;
Skin;
Electrospun nanofibrous membrane;
Gelatin;
Polycaprolactone
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2022;28(1):59-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective:For severe skin defects which are deep to dermis, engineered skin with epidermis and dermis (bilayered) is required. Based on the success of engineering epidermis with GT/PCL electrospun membranes, our study was to investigate whether this membrane could be also used for engineering bilayered skin graft.Methods:From 2013 to 2019, we first prepared three GT/PCL electrospun membranes with different proportion (70∶30; 50∶50; 30∶70) in our laboratory; the biocompatibility of the membrane was evaluated in vitro by seeding fibroblasts or keratinocytes on the membranes. Then the outcome of GT/PCL membranes repairing skin defects in the nude mouse was investigated.Results:Cell attachment and proliferation were significantly improved with increase of gelatin. Histological analyses showed that bilayered skin engineered with GT/PCL (70∶30) group could form relatively better structure after 3 weeks of cultivation in vitro. Further in vivo transplantation studies revealed that scaffolds were not degraded in all three groups, indicating that these materials were not suitable for engineering bilayered skin although they had good biocompatibility.Conclusions:The higher gelatin membranes possess better biocompatibility. Further in vivo transplantation studies reveal that bilayered skin engineered with GT/PCL membranes is able to repair skin defects in the nude mouse.
