Analysis of the laboratory detection methods and clinical characteristics in 2019-nCoV Omicron variants
10.3760/cma.j.cn114452-20220110-00015
- VernacularTitle:新型冠状病毒Omicron变异株实验室检测方法及临床特征分析
- Author:
Jianying LI
1
;
Yong CHEN
;
Meiping CHEN
;
Zhiguo ZHOU
;
Yong WU
;
Weiliang HUANG
Author Information
1. 长沙市第一医院检验科，长沙 410005
- Keywords:
2019-nCoV;
Omicron variant;
Polymerase chain reaction;
Sequence analysis;
Clinical characteristics
- From:
Chinese Journal of Laboratory Medicine
2022;45(4):360-365
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the laboratory detection methods and clinical characteristics of patients with 2019-nCoV Omicron variant infection, to realize the rapid identification and diagnosis of 2019-nCoV Omicron variants.Methods:Totally 80 overseas patients in First Hospital of Changsha from December 16 in 2021 to January 5 in 2022 were selected, the nucleic acids and mutant genes were detected by fluorescent PCR and genome sequencing, and the clinical characteristics of patients with 2019-nCoV Omicron variant infection were analyzed.Results:The specificity was 100% (58/58) and positive predictive value was 100% (21/21) respectively, the sensitivity was 95.5% (21/22), negative predictive value was 98.3% (58/59) by detected with fluorescent PCR. It was found that there were 45-50 nucleotide displacement sites in the genome and 25-30 amino acid mutation sites in S gene fragment by genome sequencing. Clinical analysis showed that mild cases were 59.1% (13/22) in layouts, without severe and critical cases. Ages were positively associated with the clinical classification (ρ=0.698, P<0.001), foundation infections were positively associated with the clinical classification (ρ=0.636, P<0.001). Conclusions:Patients with 2019-nCoV Omicron variant infection had a high viral load and long negative conversion time of nucleic acid. Ages and foundation infections were positively associated with the clinical classification. AST/ALT was higher in the early stage of the disease. Fluorescent PCR method can be used in rapid screening patients with 2019-nCoV Omicron variant infection.
