Application of digital PCR technology in the precise molecular diagnosis: opportunities and challenges
10.3760/cma.j.cn114452-20210903-00559
- VernacularTitle:数字PCR技术在精准分子诊断中的应用：机遇与挑战
- Author:
Fengxin XIE
1
;
Weidong JIN
;
Huiyan TIAN
;
Haiyan HE
;
Weiling FU
;
Yang ZHANG
Author Information
1. 陆军军医大学第一附属医院检验科，重庆 400038
- Keywords:
Digital polymerase chain reaction;
Pathogenic microorganisms;
Neoplasms;
Clinical laboratory techniques
- From:
Chinese Journal of Laboratory Medicine
2022;45(3):214-219
- CountryChina
- Language:Chinese
-
Abstract:
Digital polymerase chain reaction (dPCR) is an absolute quantitative technique that has been rapidly developed in recent years. This technique assigns the reaction system containing DNA template to a large number of independent reaction units for PCR, and calculates the DNA copy number according to the Poisson distribution and statistical positive signals. In contrast to conventional qPCR, dPCR does not depend on amplification curves, is not affected by amplification efficiency, thus has high accuracy and repeatability, and can achieve the absolute quantification. This article reviews the development history of dPCR and its application in molecular diagnosis, tumor liquid biopsy and prenatal diagnosis of infectious diseases, and looks forward to the application prospect of this technology.
