High-Fat Diet and Voluntary Chronic Aerobic Exercise Recover Altered Levels of Aging-Related Tryptophan Metabolites along the Kynurenine Pathway.
- Author:
Keon Joo LEE
1
;
Keun Hwa JUNG
;
Joo Youn CHO
;
Soon Tae LEE
;
Hwa Suk KIM
;
Jun Hwa SHIM
;
Sang Kun LEE
;
Manho KIM
;
Kon CHU
Author Information
- Publication Type:Original Article
- Keywords: tryptophan metabolites; kynurenine pathway; aging; voluntary chronic aerobic exercise; high-fat diet
- MeSH: Aged; Aging; Animals; Chromatography, Liquid; Diet, High-Fat*; Exercise*; Humans; Infant; Kynurenic Acid; Kynurenine*; Mice; Physiological Processes; Tryptophan*
- From:Experimental Neurobiology 2017;26(3):132-140
- CountryRepublic of Korea
- Language:English
- Abstract: Tryptophan metabolites regulate a variety of physiological processes, and their downstream metabolites enter the kynurenine pathway. Age-related changes of metabolites and activities of associated enzymes in this pathway are suggestable and would be potential intervention targets. Blood levels of serum tryptophan metabolites in C57BL/6 mice of different ages, ranging from 6 weeks to 10 months, were assessed using high-performance liquid chromatography, and the enzyme activities for each metabolic step were estimated using the ratio of appropriate metabolite levels. Mice were subjected to voluntary chronic aerobic exercise or high-fat diet to assess their ability to rescue age-related alterations in the kynurenine pathway. The ratio of serum kynurenic acid (KYNA) to 3-hydroxylkynurenine (3-HK) decreased with advancing age. Voluntary chronic aerobic exercise and high-fat diet rescued the decreased KYNA/3-HK ratio in the 6-month-old and 8-month-old mice groups. Tryptophan metabolites and their associated enzyme activities were significantly altered during aging, and the KYNA/3-HK ratio was a meaningful indicator of aging. Exercise and high-fat diet could potentially recover the reduction of the KYNA/3-HK ratio in the elderly.
