Antimicrobial resistance and molecular typing of Salmonella typhimurium in Wuxi city, 2011-2018
10.3760/cma.j.cn114452-20210601-00345
- VernacularTitle:2011至2018年无锡市鼠伤寒沙门菌耐药性分析与分子分型研究
- Author:
Wenting CAO
1
;
Dan SHA
;
Qianqian QI
;
Guangyuan MA
;
Hongxia GUAN
Author Information
1. 无锡市疾病预防控制中心，无锡 214023
- Keywords:
Salmonella typhimurium;
Microbial sensitivity tests;
Molecular typing
- From:
Chinese Journal of Laboratory Medicine
2022;45(2):157-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Analysis of the antibiotic resistance profiles and molecular typing of Salmonella typhimurium ( S. typhimurium) isolated in Wuxi city from 2011 to 2018. Methods:A total of 109 S. typhimurium isolates were detected from different types monitoring samples in Wuxi city from 2011 to 2018. Microbroth dilution method was used to test antimicrobial susceptibility of S. typhimurium for 17 antimicrobial agents. Pulsed field gel electrophoresis (PFGE) was used to conduct molecular typing. Statistical analysis was carried out using Chi-square test. Results:Tetracycline-resistance and ampicillin-resistance were most frequency in 109 S. typhimurium isolates, 69.72% (76/109) and 68.81% (75/109), respectively. For compound sulfamethoxazole, chloramphenicol, ciprofloxacin, ampicillin/sulbactam, azithromycin, nitrofurantoin, cefotaxime and aztreonam, the resistance rates were 23.85%(26/109), 22.02%(24/109), 11.93%(13/109), 4.59%(5/109), 3.67%(4/109), 3.67%(4/109), 0.92%(1/109), 0.92%(1/109), respectively. All isolates were susceptible to amikacin, ertapenem, imipenem, meropenem, ceftazidime and ceftazidime-avibactam. Azithromycin-resistance isolates were decreasing year by year gradually. The aztreonam and cefotaxime-resistant isolates were found since 2018, while chloramphenicol and compound sulfa-resistant isolates showed upward trend simultaneously. Conclusions:S. typhimurium in Wuxi city exhibited highly resistant to tetracycline and ampicillin. The significant variability existed between genotype and phenotype of S. typhimurium.
