Repeated Oral Administration of Human Serum Albumin Protects from the Cerebral Ischemia in Rat Brain Following MCAO.
- Author:
Hyejin PARK
1
;
Minyoung HONG
;
Gil Ja JHON
;
Youngmi LEE
;
Minah SUH
Author Information
- Publication Type:Original Article
- Keywords: Neuroprotection; Hypoxia; MCAO (Middle cerebral artery occlusion); Human serum albumin; Optical recording of intrinsic signal
- MeSH: Administration, Oral*; Animals; Anoxia; Behavior Rating Scale; Blood Volume; Brain Ischemia*; Brain*; Electric Stimulation; Half-Life; Hemodynamics; Humans*; Infarction, Middle Cerebral Artery; Ischemia; Neuroprotection; Neuroprotective Agents; Optical Imaging; Rats*; Rats, Sprague-Dawley; Rodentia; Serum Albumin*; Stroke
- From:Experimental Neurobiology 2017;26(3):151-157
- CountryRepublic of Korea
- Language:English
- Abstract: Albumin is known to have neuroprotective effects. The protein has a long half-life circulation, and its effects can therefore persist for a long time to aid in the recovery of brain ischemia. In the present study, we investigated the neuroprotective effects of human serum albumin (HSA) on brain hemodynamics. Albumin is administrated using repeated oral gavage to the rodents. Sprague-Dawley rats underwent middle cerebral artery occlusion procedures and served as a stroke model. Afterwards, 25% human serum albumin (1.25 g/kg) or saline (5 ml/kg) was orally administrated for 2 weeks in alternating days. After 2 weeks, the rodents were assessed for levels of brain ischemia. Our testing battery consists of behavioral tests and in vivo optical imaging sessions. Modified neurological severity scores (mNSS) were obtained to assess the levels of ischemia and the effects of HSA oral administration. We found that the experimental group demonstrated larger hemodynamic responses following sensory stimulation than controls that were administered with saline. HSA administration resulted in more significant changes in cerebral blood volume following direct cortical electric stimulation. In addition, the mNSS of the treatment group was lower than the control group. In particular, brain tissue staining revealed that the infarct size was also much smaller with HSA administration. This study provides support for the efficacy of HSA, and that long-term oral administration of HSA may induce neuroprotective effects against brain ischemia.
