Tumor-promoting role of Porphyromonas gingivalis fimbrillin in progression of esophageal squamous cell cancer
10.3760/cma.j.cn112309-20210704-00226
- VernacularTitle:菌毛素介导牙龈卟啉单胞菌对食管鳞癌促进作用
- Author:
Haojie RUAN
1
;
Weigang CHENG
;
Yelin JIAO
;
Pan CHEN
;
Haijun XU
;
Shegan GAO
;
Yijun QI
Author Information
1. 河南科技大学第一附属医院临床医学院，肿瘤医院，河南省微生态与食管癌防治重点实验室，河南省肿瘤表观遗传重点实验室，洛阳 471003
- Keywords:
Porphyromonas gingivalis;
Fimbrillin;
Esophageal squamous cell carcinomas;
pSmad2/3;
Proliferation;
Migration;
Invasion
- From:
Chinese Journal of Microbiology and Immunology
2022;42(4):275-280
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Porphyromonas gingivalis ( P. gingivalis) fimbrillin (FimA) on the progression of esophageal squamous cell carcinoma (ESCC). Methods:Wild-type P. gingivalis and fimA gene-deleted P. gingivalis ( fimA-/-P. gingivalis) were used to infect ESCC cells after morphology and PCR identification. Immunofluorescence, CCK-8 and Transwell chamber were used to detect the effects of FimA on the infectivity of P. gingivalis and it influences on cell invasion, proliferation and migration. Western blot was used to detect pSmad2/3 changes. The growth of tumor was detected in a nude mouse model bearing subcutaneous tumor. Results:Deletion of FimA might reduce the interbacterial adhesion of P. gingivalis. Compared with wild-type P. gingivalis, less fimA-/-P. gingivalis could infect NE6-T cells. Moreover, the proliferation, migration and invasion of NE6-T and KYSE30 cells as well as the activation of pSmad2/3 induced by P. gingivalis were inhibited after deletion of FimA. The growth of KYSE30 infected by fimA-/-P. gingivalis in nude mice was significantly slower than that of the wild-type P. gingivalis group. Conclusions:FimA mediated the effects of P. gingivalis on promoting the evolution of ESCC and was a potential target molecule to block the tumor-promoting effect of P. gingivalis.
