Characteristics of gut microbiota in hyperuricemia population based on full-length 16S rRNA sequencing
10.3760/cma.j.cn112309-20220106-00006
- VernacularTitle:基于全长16S rRNA测序的高尿酸血症人群肠道菌群特征
- Author:
Haiying JIA
1
;
Feng HU
;
Li YANG
;
Xiaoning ZOU
;
Zhen WANG
;
Lingjun MENG
;
Yerong LI
Author Information
1. 中国人民解放军战略支援部队特色医学中心特勤健康管理科，北京 100101
- Keywords:
Gut microbiota;
Hyperuricemia;
Full-length 16S rRNA sequencing
- From:
Chinese Journal of Microbiology and Immunology
2022;42(4):265-274
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the differences in gut microbiota between patients with hyperuricemia (HUA) and healthy population for better understanding the correlation between gut microflora and high uric acid.Methods:This study recruited 63 adult volunteers, including 25 HUA patients (HUA group) and 38 healthy people (control group), who underwent physical examination in the PLA Strategic Support Force Characteristic Medical Center in 2021. Their fecal samples were collected and analyzed by 16S rRNA high-throughput full-length gene sequencing to analyze the composition of gut microbiota.Results:The overall composition of gut microbiota was different between HUA group and control group. The α diversity index decreased significantly in HUA group and β diversity analysis showed that there were significant differences between the two groups. HUA group showed increased Bacteroidetes and decreased Firmicutes. LEfSe analysis indicated a unique microbiota structure in HUA group, characterized by significantly decreased short-chain fatty acid (SCFA)-producing bacteria represented by Faecalibacterium prausnitzii and significantly increased Streptococcus salivarius, Bacteroides fragilis, Fusobacterium hwasookii, Flavonifractor plautii, Mycobacterium mucogenicum B and Blautia sp003287895. In addition, functional prediction through PICRUSt2 showed that the metabolism related to gut microbiota SCFA pathway in HUA group was decreased, which was consistent to the unique microbiota structure. Conclusions:Compared with healthy population, patients with hyperuricemia had different composition of gut microbiota and metabolic feature.
