Adaptive phenotypes of Yersinia pestis induced by successive passages in macrophages
10.3760/cma.j.cn112309-20220211-00042
- VernacularTitle:巨噬细胞内连续传代诱导鼠疫耶尔森菌表型变化的研究
- Author:
Xin CHEN
1
;
Kai SONG
;
Yarong WU
;
Liting XIAO
;
Junyan JIN
;
Yipu DU
;
Yujun CUI
;
Li YU
;
Yajun SONG
Author Information
1. 安徽医科大学基础医学院，合肥 230032
- Keywords:
Yersinia pestis;
Macrophages;
Adaptive changes;
Virulence
- From:
Chinese Journal of Microbiology and Immunology
2022;42(4):251-257
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes in adaptive phenotypes of Yersinia pestis ( Yp) during successive passages in macrophages. Methods:A Yp strain of 201-MI was induced by 50 successive passages of Yp 201 strain in Raw264.7 cells. Phenotypic characteristics of 201 and 201-MI strains were compared by analyzing their survival rates in macrophages, growth curves, biofilm formation abilities, acid and hydrogen peroxide-stress tolerance, and virulence to mammal cells (Raw264.7 and HeLa cells) and mice. Results:Comparing with 201 strain, 201-MI strain showed various phenotypic changes, including higher survival rate in Raw264.7 cells, faster growth in iron-deficient medium, higher tolerance to acid and hydrogen peroxide, decreased biofilm formation ability, and less damages to Raw264.7 and HeLa cells. More-over, 201-MI strain showed decreased virulence to mice in both subcutaneous and intraperitoneal challenges. Preliminary comparative genomics analysis revealed some indel and nonsense mutations in 201-MI strain, which might account for its phenotype changes.Conclusions:After successive passages in macrophages, Yp showed some phenotypic changes, which might reflect its adaptive evolution under the pressure of macrophages. Detailed multi-omics analysis would be of great help to understand the underlying genetic mechanisms of these changes, and the related Yp-macrophage interaction processes as well.
