Chlamydia muridarum respiratory infection induces macrophage infiltration and polarization toward M1 phenotype
10.3760/cma.j.cn112309-20210608-00200
- VernacularTitle:沙眼衣原体小鼠肺炎株呼吸道感染诱导巨噬细胞浸润并向M1极化
- Author:
Yueyue XU
1
;
Wenhao NIU
;
Ye JING
;
Xiaoyu ZHA
;
Jiajia ZENG
;
Shuaini YANG
;
Tongxing QU
;
Hong ZHANG
;
Hong BAI
Author Information
1. 天津医科大学基础医学院免疫学系，国家教育部免疫微环境与疾病重点实验室，天津市细胞和分子免疫学重点实验室，天津 300070
- Keywords:
Chlamydia muridarum;
Macrophage;
Polarization;
Inflammatory response
- From:
Chinese Journal of Microbiology and Immunology
2022;42(3):194-201
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the infiltration and polarization of macrophages in mice during Chlamydia muridarum ( Cm) respiratory infection. Methods:C57BL/6 mice were intranasally infected with 1×10 3 inclusion-forming units (IFU) of Cm to establish the mouse model of Cm respiratory tract infection. The percentages of CD45 + F4/80 + cells and the macrophages expressing CD86, major histocompatibility complex Ⅱ (MHC), inducible nitric oxide synthase (iNOS) and CD206 were detected by flow cytometry. Expression of iNOS, CD206 and CCL2 at mRNA level was detected by real-time quantitative PCR. Results:Cm respiratory tract infection induced the increase of macrophages in mouse lung tissues. Compared with uninfected group, CD45 + F4/80 + macrophages were increased significantly from day 3 and reached the peak on day 7 after Cm infection. Moreover, the expression of CD86, MHCⅡ and CCL2 was increased, and the macrophages were polarized to M1 phenotype. However, the expression of M2 macrophage marker CD206 was decreased gradually. Further studies showed that iNOS expression, the indicator of M1 macrophage activation, was increased after Cm infection and reached to the top on day 7. Conclusions:Cm respiratory infection could induce the infiltration of macrophages in lung tissues and promote the polarization of macrophages to M1 phenotype.
