SARS-CoV-2 membrane protein is incompetent at inducing antibody-dependent enhancement though Fc receptor pathway
10.3760/cma.j.cn112309-20211201-00410
- VernacularTitle:新型冠状病毒膜蛋白不具有以Fc受体途径诱导抗体依赖性增强效应的能力
- Author:
Penglan CHANG
1
;
Qihan LI
;
Dandan LI
;
Suqin DUAN
;
Xueqi LI
;
Jingjing ZHANG
;
Jingfu ZENG
;
Ying ZHANG
Author Information
1. 中国医学科学院北京协和医学院医学生物学研究所，云南省重大传染病疫苗研究室，昆明 650118
- Keywords:
SARS-CoV-2;
Antibody dependent enhancement effect;
Membrane protein;
Non-neutralizing antibody
- From:
Chinese Journal of Microbiology and Immunology
2022;42(3):161-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the immune characteristics of SARS-CoV-2 membrane (M) protein, especially the possibility of inducing antibody-dependent enhancement effect (ADE).Methods:Full-length SARS-CoV-2 M protein was prepared by prokaryotic expression system and purified. BALB/c mice were immunized subcutaneously three times (on day 1, day 14 and day 21) by purified M protein. Serum samples were collected before immunization and after each immunization. The specificity of immune sera against M protein was identified by Western blot, and the antibody titers were detected by ELISA and neutralization test. In the presence of anti-M protein serum, the proliferation of SARS-CoV-2 in dendritic cells, nature killer cells, T and B cells was detected in vitro. Results:The immune sera from BALB/c mice immunized with purified full-length M protein of SARS-CoV-2 specifically recognized viral M protein. The titer of anti-whole virus antibody in immune sera was about 1∶400, but the antibody could not neutralize live virus. Moreover, the antibody could not help the virus to infect and proliferate in the various types of immune cells with Fc receptor (FcR).Conclusions:Non-neutralizing antibody induced by M protein could not cause ADE through FcR pathway.
