HIV-1 genetic characteristics and drug resistance in newly diagnosed population in Baoding city of Hebei Province
10.3760/cma.j.cn112309-20210814-00272
- VernacularTitle:河北省保定市HIV-1感染者毒株基因亚型和耐药研究
- Author:
Weiguang FAN
1
;
Ying XING
;
Leilei HAN
;
Miaomiao SU
;
Juan MENG
;
Erhei DAI
;
Penghui SHI
;
Hanping LI
Author Information
1. 河北省保定市人民医院检验科，保定 071000
- Keywords:
HIV-1;
Subtypes;
Resistance mutations
- From:
Chinese Journal of Microbiology and Immunology
2022;42(2):88-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the distribution of HIV-1 genotypes and drug resistance in newly diagnosed HIV-1 patients in Baoding in 2020.Methods:A self-developed method was used to amplify the pol gene sequence of HIV-1, and the sequencing results were analyzed by phylogenetic analysis and compared with the Stanford drug resistance database to determine the HIV-1 subtypes and gene mutations. Results:A total of 96 patients with HIV-1 infection were recruited in this study, and 83 pol gene sequences were successfully obtained. In the study population, 88 (91.7%) were male with an average age of 39 years and 54 (56.3%) were married. Most of the patients were infected through sexual contact (95.8%, 92/96), and 75.0% (72/96) were through homosexual transmission. Phylogenetic analysis showed that various HIV-1 subtypes were detected and among them, CRF01_AE (51.8%, 43/83), CRF07_BC (24.1%, 20/83) and B subtype (10.8%, 9/83) were the most epidemic strains. Moreover, the subtypes of newly identified recombinant strains in recent years accounted for 13.3% (11/83). Drug resistance test results showed that the pre-treatment drug resistance rate in newly diagnosed HIV-1 patients was 8.4% (7/83), and the drug resistance rates to protease inhibitor (PIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs) and integrase inhibitors (INIs) were 3.6% (3/83), 1.2% (1/83) and 3.6% (3/83), respectively. Conclusions:The HIV-1 subtypes in the newly diagnosed population in Baoding in 2020 were complex and diverse. There were many unique recombinant strains and drug-resistant strains. Therefore, it was necessary to strengthen drug resistance monitoring as well as the prevention and control of HIV-1 infection in this area.
