Transcriptional regulation of pilABCD by OpaR in Vibrio parahaemolyticus
10.3760/cma.j.cn112309-20210729-00248
- VernacularTitle:OpaR对副溶血弧菌 pilABCD操纵子的调控研究
- Author:
Renfei LU
1
;
Junfang SUN
;
Xingfan XUE
;
Miaomiao ZHANG
;
Xue LI
;
Qimin WU
;
Yiquan ZHANG
Author Information
1. 南通市第三人民医院检验科 212001
- Keywords:
Vibrio parahaemolyticus;
Quorum sensing;
Transcriptional regulation;
OpaR;
pilABCD
- From:
Chinese Journal of Microbiology and Immunology
2021;41(12):906-911
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the transcriptional regulation of pilABCD by the master quorum sensing (QS) regulator OpaR in Vibrio parahaemolyticus. Methods:Total RNAs were extracted from the wild type (WT) and opaR mutant (Δ opaR) strain. Quantitative real-time PCR (qPCR) was employed to calculate the transcriptional variation of pilA (the first gene of pilABCD operon) between WT and Δ opaR. The regulatory DNA region of pilABCD was cloned into the corresponding restriction endonuclease sites of pHRP309 harboring a promoterless lacZ reporter gene. The recombinant pHRP309 plasmid was then transferred into WT and Δ opaR, respectively, to detect the β-galactosidase activity in cellular extracts using a β-Galactosidase Enzyme Assay System (Promega). The primer extension assay was applied to map the transcription start site of pilABCD using the total RNAs extracted from the WT strain as the template. The regulatory DNA region of pilABCD was amplified by PCR, and the over-expressed His-OpaR was purified under native conditions with nickel loaded HiTrap Chelating Sepharose columns (Amersham). Thereafter, the electrophoretic mobility shift assay (EMSA) was applied to analyze the DNA-binding activity of His-OpaR to the target DNA in vitro, and the DNase I footprinting assay was further employed to detect the DNA-binding sites of His-OpaR within the target DNA. Results:The results of qPCR and LacZ fusion assays showed that OpaR activated the transcription of pilABCD, leading to a gradual increase in the expression level of pilA with the extension of culture time. The primer extension assay detected only one transcription start site located at 155 bp upstream of pilA. The results of EMSA and DNase Ⅰ footprinting assays showed that His-OpaR protected two DNA regions located from -246 to -197 bp and -181 to -131 bp upstream of pilA. Conclusions:Vibrio parahaemolyticus OpaR activated the transcription of pilABCD in a direct manner.
