Expression of miR-128-3p in gastric cancer and its effect on migration and invasion of gastric cancer cells
10.3760/cma.j.cn113855-20210915-00553
- VernacularTitle:miR-128-3p在胃癌组织中的表达及其对胃癌细胞迁移和侵袭能力的影响
- Author:
Kelei HUA
1
;
Yingkun REN
;
Mingke HUO
;
Zhichuang DONG
Author Information
1. 郑州大学附属肿瘤医院河南省肿瘤医院普外科，郑州 450008
- Keywords:
Stomach neoplasms;
Cell migration assays;
Neoplasm invasiveness
- From:
Chinese Journal of General Surgery
2022;37(4):279-283
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of miR-128-3p on the migration and invasion of the gastric cancer cells.Methods:qRT-PCR was used to detect the expression of miR-128-3p in 126 gastric cancer tissues and adjacent tissues from Jan 2014 to Jan 2016 at He'nan Cancer Hospital. The effect of miR-128-3p on the invasion and migration of gastric cancer cell line was detected.The expression of miR-128-3p related proteins was detected by Western blotting, miRNA on-line target prediction tool for the prediction of miR-128-3p directly regulated downstream target genes.Results:the expression of miR-128-3p in gastric cancer was significantly higher than that in adjacent non-tumor tissues ( P<0.05). The expression of miR-128-3p was correlated with the vascular tumor thrombus, pN staging and pTNM staging, the prognosis of patients with high expression of miR-128-3p was poor (all P<0.05). MiR-128-3p expression was significantly higher in gastric cancer cell lines ( P<0.05). Online target prediction tool and double luciferase reporter gene showed that CLDN18 was a downstream target gene directly regulated by mir-128-3p. Conclusion:The high expression of miR-128-3p is related to the poor prognosis of gastric cancer patients.
