ALDH1A3 + INS + cells in non-diabetic subjects and risk for type 2 diabetes: Analysis of cross-sectional data
10.3760/cma.j.cn311282-20211112-00721
- VernacularTitle:非糖尿病人群中ALDH1A3 +INS +细胞与2型糖尿病风险因素的相关性研究
- Author:
Tengli LIU
1
;
Rui LIANG
;
Peng SUN
;
Le WANG
;
Na LIU
;
Jiaqi ZOU
;
Yaojuan LIU
;
Xuejie DING
;
Shusen WANG
Author Information
1. 天津市笫一中心医院器官移植中心 300192
- Keywords:
Diabetes mellitus, type 2;
β cell dedifferentiation;
Total cholesterol;
Glycated hemoglobin
- From:
Chinese Journal of Endocrinology and Metabolism
2022;38(3):219-224
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the correlations of β cell dedifferentiation in non-diabetic subjects with risk factors for type 2 diabetes mellitus(T2DM).Methods:Immunofluorescence staining with insulin and β cell dedifferentiated marker ALDH1A3 was used to evaluate the β cell dedifferentiation levels in 38 non-diabetic and 23 T2DM. Correlation analyses were performed between β cell dedifferentiation levels and available clinical parameters including age, body mass index, HbA 1C level, triglycerides, and cholesterol levels in non-diabetic subjects. Results:β cell dedifferentiation level defined by the positive expression of ALDH1A3 in β cells(ALDH1A3 + INS + cell proportion) was significantly elevated in T2DM subjects( P<0.001). In PreD subjects, ALDH1A3 + INS + cells proportion were decreased( P=0.050) and negatively correlated with HbA 1C( r=-0.44, P=0.006), but not with age and body mass index. The analysis of correlation with lipidemic parameters showed that ALDH1A3 + INS + cells proportion was positively correlated with plasma total cholesterol level( r=0.39, P=0.045), but not plasma total triglyceride. Conclusion:ALDH1A3 + INS + cells were found to be decreased in prediabetes, suggesting that there may be enhanced β-cell identity in prediabetes to compensate for insulin secretion requirements; ALDH1A3 + INS + cells were elevated in people with high plasma total cholesterol levels, suggesting that total cholesterol may be one of the factors that induce β-cell dedifferentiation.
