Vitamin D supplementation improves testicular function in aging rats induced by D-galactose
10.3760/cma.j.cn311282-20210705-00429
- VernacularTitle:补充维生素D对D-半乳糖诱导的衰老大鼠睾丸功能的改善作用及机制
- Author:
Danqing LIU
1
;
Ying CUI
;
Yanling LIU
;
Feng GUO
;
Peijie DU
;
Mingwei SHAO
;
Yanxia LIU
;
Guijun QIN
Author Information
1. 郑州大学第一附属医院内分泌科 450052
- Keywords:
Vitamin D;
D-galactose;
Aging;
Testicular function;
Nrf2-ARE pathway
- From:
Chinese Journal of Endocrinology and Metabolism
2022;38(2):151-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects and potential mechanism of vitamin D supplementation on testicular function in aging rats induced by D-galactose.Methods:The aging rats were induced by D-galactose with subcutaneous injection. The animals were randomly divided into 6 groups: aging rats (DG), aging rats with low-dose vitamin D supplementation (LD), aging rats with high-dose vitamin D supplementation (HD), normal control rats(NC), normal rats with low-dose vitamin D supplementation(LN), normal rats with high-dose vitamin D supplementation (HN). The body weight, testicular weight, serum testosterone concentrations and sperm quality of the rats in each group were measured. The testis morphological changes were detected using light microscopy. The activity of superoxide dismutase (SOD) and level of malondialdehyde(MDA) were determined with spectrophotometer. The expression levels of Nrf2, GCLC, SOD2 and VDR in testis were detected by western blot.Results:At baseline, compared with NC group, testicular weight, serum testosterone level, SOD activity, Nrf2, GCLC and SOD2 expression levels were significantly decreased in DG group, while MDA level was significantly increased. After vitamin D supplementation, testicular weight, testosterone levels and SOD activity in both of HD and LD groups were significantly increased, while the MDA level was significantly decreased. The expression levels of Nrf2, GCLC, SOD2 and VDR were significantly increased.Conclusion:Vitamin D supplementation may enhance the testicular antioxidant capacity through activating Nrf2-ARE signaling pathway, and improve the testicular function in D-galactose-induced aging rats.
