High glucose promotes the release of IL-1β and IL-18 from placental trophoblast by activating NLRP3 inflammasome
10.3760/cma.j.cn311282-20210312-00158
- VernacularTitle:高糖通过激活NLRP3炎症小体促进胎盘滋养细胞IL-1β、IL-18的释放
- Author:
Tianxiao HU
1
;
Xiujing WANG
;
Yun RUAN
;
Jiaqi YAO
;
Fengmei WANG
;
Yongjun XU
;
Jing WANG
;
Qingying TAN
Author Information
1. 浙江中医药大学，生命科学学院，杭州 310053
- Keywords:
Gestational diabetes mellitus;
NLRP3 inflammasome;
Interleukin-1β;
Interleukin-18
- From:
Chinese Journal of Endocrinology and Metabolism
2022;38(1):36-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of high glucose on the release of interleukin (IL)-1β and IL-18 in placental trophoblast by activating NLRP3 inflammasome.Methods:Gestational diabetes mellitus(GDM) placentas and control placentas were collected and the expression levels of NLRP3 and Caspase-1 were determined. Human placental trophoblast HTR-8/SVneo were cultured and divided into control group(5.5 mmol/L glucose), high glucose group(25 mmol/L glucose), DMSO+ high glucose group, and Ac-YVAD-cmk(NLRP3 inflammasome inhibitor)+ high glucose group. The expression levels of NLRP3 and Caspase-1 in cells as well as the contents of IL-1β and IL-18 in the medium were determined.Results:The expression levels of NLRP3 and Caspase-1 in GDM placenta were higher than those in control placenta( P<0.05) and positively correlated with homeostasis model assessment of insulin resistant index(HOMA-IR) and fasting insulin. The expression levels of NLRP3 and Caspase-1 in HTR-8/SVneo cells and the secretion levels of IL-1β and IL-18 in high glucose group were higher than those in control group( P<0.05). Ac-YVAD-cmk significantly suppressed high glucose-stimulated IL-1β and IL-18 secretion( P<0.05). Conclusion:High glucose promotes the release of IL-1β and IL-18 from placental trophoblast via activating NLRP3 inflammasome.
