Effect of electrical stimulation on lipopolysaccharide-induced activation of M1 microglia
10.3760/cma.j.cn131073.20211021.00303
- VernacularTitle:电刺激对LPS诱导M1型小胶质细胞活化的影响
- Author:
Wenjie LIU
1
;
Ye WANG
;
Rui DONG
;
Mingshan WANG
;
Gaofeng ZHANG
;
Kaiyue SHAN
;
Huai-Long CHEN
Author Information
1. 青岛大学附属青岛市市立医院麻醉科，青岛 266071
- Keywords:
Electrical stimulation;
Lipopolysaccharides;
Microglia
- From:
Chinese Journal of Anesthesiology
2022;42(3):265-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of electrical stimulation on lipopolysaccharide (LPS)-induced activation of M1 microglia.Methods:The well-growing BV2 microglia cells were divided into 3 groups ( n=18 each) using a random number table method: control group (group C), group LPS, LPS and electrical stimulation group (group LE). The cells were cultured for 24 h in normal culture atmosphere in group C. In group LPS and group LE, the LPS medium culture 100 ng/ml was added, and the cells were cultured for 24 h. In group LE, cells were stimulated with 100 mV/mm direct current for 4 h before LPS incubation.The levels of tumor necrosis factor-α (TNF-α) and leukocyte interleukin-1β (IL-1β) were determined by enzyme-linked immunosorbent assay.The expression of the M1 microglia surface markers CD32 and inducible nitric oxide synase (iNOS) was detected using immunofluorescent staining.The expression of CD32 and iNOS mRNA was detected using quantitative real-time polymerase chain reaction. Results:Compared with group C, the concentrations of TNF-α and IL-1β were significantly increased, and the expression of CD32 and iNOS protein and mRNA was up-regulated in LPS and LE groups ( P<0.05). Compared with group LPS, the concentrations of TNF-α and IL-1β were significantly decreased, and the expression of CD32 and iNOS protein and mRNA was down-regulated in group LE ( P<0.05). Conclusions:Electrical stimulation can inhibit LPS-induced activation of M1 microglia and thus alleviate the inflammatory responses.
