Effect of propofol on proliferation of neural stem cells in mice and the role of Sp1-EGFR-Akt signaling pathway
10.3760/cma.j.cn131073.20210827.00110
- VernacularTitle:异丙酚对小鼠神经干细胞增殖的影响及Sp1-EGFR-Akt信号通路在其中的作用
- Author:
Liangtian LAN
1
;
Ming WEI
;
Dihan LU
;
Keyu CHEN
;
Xia FENG
Author Information
1. 中山大学附属第一医院麻醉科，广州 510080
- Keywords:
Propofol;
Neural stem cells;
Cell proliferation;
Receptor, epidermal growth factor;
Protein-serine-threonine kinases;
Sp1 transcription factor
- From:
Chinese Journal of Anesthesiology
2022;42(1):50-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of propofol on proliferation of neural stem cells (NSCs) in mice and the role of specificity protein-1 (Sp-1)-epidermal growth factor receptor (EGFR)-protein kinase B (Akt) signaling pathway.Methods:Primary NSCs harvested from both the cortices and hippocampus of C57BL/6 mouse embryos were identified by immunofluorescent staining of Nestin.NSCs at passages 3-6 were divided into 3 groups ( n=21 each) using a random number table method: normal saline control group (C group), propofol group (P group) and propofol plus Sp1 inhibitor plicamycin group (PP group). Propofol at a final concentration of 10 μmol/L was added in group P. Propofol at a final concentration of 10 μmol/L and plicamycin at a final concentration of 100 nmol/L were added in group PP.The equal volume of normal saline was added in group C. The medium was replaced after 6 h of incubation and the cells were continuously incubated.The proliferation of NSCs was assessed by direct cell counting at 24, 36, 48, 60 and 72 h after the end of treatment with drugs.At 6 h after the end of treatment with drugs, the expression of Sp1 and EGFR mRNA was detected by real-time fluorescent quantitative polymerase chain reaction, and the expression of Sp1, Akt and phosphorylated Akt (p-Akt) by Western blot. Results:Compared with group C, the count of NSCs was significantly increased at 48, 60 and 72 h after treatment with drugs, and the expression of EGFR mRNA, Sp1 protein and mRNA and p-Akt was up-regulated in group P ( P<0.05 or 0.01), and no significant change was found in each parameter in group PP ( P>0.05). Compared with group P, the count of NSCs was significantly decreased at 48 and 60 h after treatment with drugs, and the expression of EGFR protein and mRNA and p-Akt was down-regulated in group PP ( P<0.05 or 0.01). Conclusions:Propofol can promote the proliferation of NSCs, and the mechanism may be related to activation of Sp1-EGFR-Akt signaling pathway in mice.
