Effects of different density rat fibroblasts on expression of Cx43 in cardiomyocytes and cell viability
10.3760/cma.j.cn131073.20210809.01227
- VernacularTitle:不同密度大鼠成纤维细胞对心肌细胞Cx43及细胞活力的影响
- Author:
Xueyan WU
1
;
Hong GAO
;
Fuxue MENG
;
Yurong FENG
;
Zhenying NIU
;
Guilong WANG
;
Ying CAO
;
Jing YI
;
Rui TONG
;
Li AN
;
Shengyan TAI
Author Information
1. 贵州医科大学麻醉学院，贵阳 550004
- Keywords:
Fibroblasts;
Myocytes, cardiac;
Connexins 43
- From:
Chinese Journal of Anesthesiology
2021;41(12):1523-1527
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of different density rat fibroblasts on the expression of conjunctin 43 (Cx43) in cardiomyocytes and cell viability.Methods:Cardiomyocytes and fibroblasts were co-cultured using Transwell, cardiomyocytes were inoculated into the lower chamber of Transwell and fibroblasts into the upper chamber of Transwell.The cells were divided into 3 groups ( n=12 each) by a random number table method: fibroblast density 0.5×10 5 cells/ml group (group C 0.5), fibroblast density 1×10 5 cells/ml group (group C 1), and fibroblast density 2×10 5 cells/ml group (group C 2), with the density of cardiomyocytes 1×10 5 cells/ml in three groups.Cardiomyocytes and fibroblasts were co-cultured for 20 h in three groups.Cardiomyocytes were collected after co-culture for determination of cell viability (by CCK8 method), apoptosis rate (by flow cytometry), and expression of Cx43 mRNA (by quantitative real-time polymerase chain reaction) and expression of Cx43 and phosphorylated Cx43 (p-Cx43) (by Western blot). Results:There was no significant difference in the apoptosis rate of cardiomyocytes among the three groups ( P>0.05). Compared with group C 0.5, the expression of Cx43 protein and mRNA and p-Cx43 was significantly up-regulated in group C 1, the cardiomyocyte viability was significantly increased, and the expression of Cx43 protein and mRNA and p-Cx43 was up-regulated in group C 2 ( P<0.05). Compared with group C 1, the cardiomyocyte viability was significantly increased, and the expression of Cx43 protein and mRNA and p-Cx43 was up-regulated in group C 2 ( P<0.05). Conclusion:Rat fibroblasts up-regulate the expression of Cx43 and enhance the activity of Cx43 in cardiomyocytes and enhance cell viability in a density-dependent manner in a certain range.
