Catalase and PPAR-gamma Polymorphisms of Rheumatoid Arthritis in Korean.
- Author:
Chan Bum CHOI
1
;
Ahmed El SOHEMY
;
Sang Seokg SEONG
;
Yong Wook PARK
;
Hye Soon LEE
;
Wan Sik UHM
;
Tae Hwan KIM
;
Jae Bum JUN
;
Dae Hyun YOO
;
Sang Cheol BAE
Author Information
1. Department of Internal Medicine, The Hospital for Rheumatic Diseases, Hanyang University, Seoul, Korea. scbae@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Rheumatoid Arthritis;
Catalase;
PPAR-gamma;
Polymorphism
- MeSH:
Arthritis, Rheumatoid*;
Catalase*;
Classification;
DNA;
HLA-DRB1 Chains;
Humans;
Korea;
Peroxisomes;
Polymorphism, Genetic;
Polymorphism, Single Nucleotide
- From:The Journal of the Korean Rheumatism Association
2004;11(3):217-226
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the association between a single nucleotide polymorphism in the catalase and peroxisome proliferator activated receptor- gamma (PPAR-gamma) genes with risk and severity of rheumatoid arthritis (RA) in Korea. METHODS: DNA was isolated from blood samples collected from 473 Korean patients with RA and 400 Korean controls. Genotyping for the C-262T polymorphism of catalase and the Pro12Ala polymorphism of PPAR-gamma was performed by PCR-RFLP (restriction fragment length polymorphism) analysis. Subjects were classified according to ACR criteria for RA, KHAQ, and radiological severity classification by Steinbroker. RESULTS: In patient group, catalase C/T and T/T polymorphism was seen in 23 (4.9%) and 0 patients while Pro/Ala and Ala/Ala PPAR-gamma polymorphism was seen in 42 (8.9%) and 1 (0.2%) patients. In control group, catalase C/T and T/T polymorphism was seen in 25 (6.3%) and 1 (0.2%) controls while Pro/Ala and Ala/Ala PPAR-gamma polymorphism was seen in 34 (8.5%) and 4 (1%) controls. Catalase and PPAR-gamma polymorphism showed no association with the susceptibility to RA individually but, when combined association was found although it had no statistical significance, which was possibly due to its low frequency. Catalase and HLA-DRB1 polymorphism showed significant interaction on development of RA but, no interaction was found between PPAR-gamma and HLA-DRB1. Catalase and PPAR-gamma polymorphism showed no association with the severity or functional status of RA. CONCLUSION: Our results show that genetic polymorphisms of catalase and PPAR-gamma has no association with the susceptibility to RA. Catalase and HLA-DRB1 polymorphism showed significant interaction on development of RA.
