The effects of WNT1 inducible signaling pathway protein 2 on hepatocyte lipid metabolism and associated mechanisms
10.3760/cma.j.issn.0254-9026.2022.04.021
- VernacularTitle:WNT1诱导信号通路蛋白2对肝细胞脂质代谢的影响及其作用机制
- Author:
Min MAO
1
;
Yalan DENG
;
Huan GONG
;
Yitian XU
;
Ruomei QI
;
Chunfeng YUN
;
Beidong CHEN
Author Information
1. 北京医院 国家老年医学中心 国家卫生健康委员会北京老年医学研究所 国家卫生健康委员会老年医学重点实验室 中国医学科学院老年医学研究所，北京 100730
- Keywords:
Hepatocytes;
WNTI inducible signaling pathway protein 2;
Triglycerides;
Cholesterol;
Lipid metabolism
- From:
Chinese Journal of Geriatrics
2022;41(4):466-471
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of various concentrations of recombinant human WISP2 protein(WISP2)on lipid metabolism in HepG2 cells.Methods:HepG2 cells were treated with different concentrations(0, 0.4, 1 and 2 μg/L)of recombinant human WISP2 for 48 hours.Cell viability was detected by Cell-Titer, and enzymatic hydrolysis methods were used to measure intracellular triacylglycerol(TG)and total cholesterol(TC)levels.The mRNA expression was detected by quantitative real-time reverse transcription-PCR(RT-qPCR)and protein expression in HepG2 cells was detected by western blot.Results:Compared with the control group, the WISP2 groups treated with various concentration did not significantly reduce the viability of HepG2 cells.TG and TC in HepG2 cells were significantly increased by recombinant human WISP2 treatment(all P<0.05).The concentrations of TG in the 0.4, 1 and 2 μg/L recombinant human WISP2-treated groups were 1.254±0.039, 1.216±0.028 and 1.174±0.014)times the concentration in the untreated group, respectively( F=6.791, P=0.006).The concentration of TC in the untreated group was 1.264±0.057, 1.394±0.101 and 1.392±0.077), respectively, times the concentration in each of the treated groups( F=7.045, P=0.005).Further experiments found that the mRNA expression of sterol regulatory element binding protein 1(SREBP1), 3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGCR), acetyl-CoA carboxylase(ACC), type 2 diacylglycerol acyltransferase(DGAT2)and the protein expression of SREBP1, ACC and fatty acid synthase(FAS)were significantly increased in the recombinant human WISP2-treated groups, compared with the control group(all P<0.05).However, the expression of lipid transporters such as the low-density lipoprotein receptor(LDLR), ApoB and ApoE and adipose triglyceride lipase(ATGL), a key lipolysis protein, was not significantly affected. Conclusions:Human recombinant WISP2 protein increases lipid levels in hepatocytes and the key underlying mechanisms may be through promoting lipid synthesis.
