Effect of upregulated HuR gene on radiosensitivity of esophageal squamous cell carcinoma cell Kyse450
10.3760/cma.j.cn113030-20210304-00086
- VernacularTitle:上调 HuR基因对食管鳞癌细胞Kyse450放射敏感性的影响
- Author:
Dan HAN
1
;
Lu LI
;
Zhiwen KAN
;
Zhenchao TAO
;
Liting QIAN
Author Information
1. 中国科学技术大学生命科学与医学部，合肥 230026
- Keywords:
HuR;
Esophageal squamous cell carcinoma;
Radiosensitivity
- From:
Chinese Journal of Radiation Oncology
2022;31(5):456-461
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of upregulation of HuR gene on the radiosensitivity of esophageal squamous cell carcinoma cell Kyse450. Methods:The HuR gene of Kyse450 cells was upregulated by lentivirus. At the same time, X-ray irradiation at a dose of 6 Gy was selected as the intervention condition. Western blot and qPCR were used to detect the expression levels of protein and RNA after Kyse450 transfection, respectively. CCK8 kit was employed to determine the cell proliferation rate. Clone formation assay was adopted to evaluate the ability of cell clone formation. Wound healing experiment and the Transwell test were performed to detect changes in cell migration. Results:CCK8 assay showed that the proliferation ability of cells was enhanced after upregulation of HuR gene, and this enhancement trend was more obvious after radiation. The plate cloning experiment showed that with the increase of radiation dose, the clone formation rates were decreased in both groups, but the clone formation rate in the overexpression group was higher than that in the control group. Wound healing experiment and Transwell test demonstrated that the wound healing rate and migration ability in the overexpression group were higher than those in the control group, and the difference was more significant after radiotherapy. Western blot showed that the levels of MMP9 and MMP2 at 24 h after radiotherapy in the overexpression group were higher than those in the control group. Conclusion:The upregulation of HuR can enhance the proliferation, cloning, migration capabilities and decrease the radiosensitivity of esophageal squamous cell carcinoma cells.
