The role of miR-21 in Endostar combined with X-ray irradiation of cardiac fibroblasts
10.3760/cma.j.cn113030-20210130-00050
- VernacularTitle:miR-21在恩度联合X线照射心肌纤维母细胞的作用
- Author:
Dongdong CAO
1
;
Weiwei OUYANG
;
Xing ZHAO
;
Shengfa SU
;
Jun ZHANG
;
Shimei FU
;
Zhangxin FENG
;
Qingsong LI
;
Zhu MA
;
Wengang YANG
;
Xiaxia CHEN
;
Bing LU
Author Information
1. 贵州医科大学附属医院/贵州医科大学附属肿瘤医院肿瘤科，贵阳 550004
- Keywords:
miR-21 gene;
Endostar;
Cardiac fibroblast;
X-ray irradiation
- From:
Chinese Journal of Radiation Oncology
2022;31(4):370-375
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the implication of micro RNA-21(miR-21) in Endostar combined with X-ray irradiation of cardiac fibroblasts (CF).Methods:Rat CFs were used in this experiment and been divided into the blank control group, 10 Gy X-ray irradiation group, Endostar group, 10 Gy X-ray+ Endostar group, 10 Gy X-ray+ Endostar+ NC mimic group (negative control 1), 10 Gy X-ray+ Endostar+ miR-21 mimic group, 10 Gy X-ray+ Endostar+ NC inhibitor group (negative control 2) and 10 Gy X-ray+ Endostar+ miR-21 inhibitor group. The proliferation of CF was determined by Methyl thiazolyl tetrazolium (MTT) assay. The expression level of Collagen Ⅰ protein was analyzed by Western blot. The expression levels of Collagen Ⅰ and miR-21 mRNA were assayed by real-time quantitative polymerase chain reaction (q-PCR).Results:In the 10 Gy X-ray+ Endostar+ miR-21 mimic group, the CF proliferation, Collagen Ⅰ and miR-21 mRNA were increased significantly compared with those in the blank control group, 10 Gy X-ray+ Endostar group, and negative control group 1 (all P<0.05). In the 10 Gy X-ray+ Endostar+ miR-21 inhibitor group, the CF proliferation and expression levels of Collagen Ⅰ mRNA were decreased significantly compared with those in the blank control group, 10 Gy X-ray+ Endostar group and negative control group 2(all P<0.05). Conclusions:The CF proliferation and Collagen Ⅰ expression are increased when the expression level of miR-21 gene is simulated. When inhibiting the expression of miR-21 gene, the CF proliferation and Collagen Ⅰ expression are reduced.
