Evaluation of the effect of 2-aminoethoxydiphenyl-borate on cardiac dysfunction in arsenic poisoning rats by stratified strain technique
10.3760/cma.j.cn131148-20211018-00746
- VernacularTitle:分层应变技术评价2-氨基乙酯二苯基硼酸对砷中毒致大鼠心功能障碍的保护作用
- Author:
Jia FENG
1
;
Wenjuan QIN
;
Zhen WANG
;
Zijing ZHAI
;
Ruimeng TIAN
;
Guilin LU
Author Information
1. 石河子大学医学院第一附属医院超声医学科，石河子 832008
- Keywords:
Stratified strain technique;
2-aminoethoxydiphenyl-borate;
Arsenic poisoning;
Rats;
Cardiac function
- From:
Chinese Journal of Ultrasonography
2022;31(5):433-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of 2-aminoethoxydiphenyl-borate (2-APB) on cardiac dysfunction in arsenic poisoning rats by stratified strain technique.Methods:Thirty-two 12-week-old SD rats were randomly divided into control group ( n=8) and arsenic exposure group ( n=24). After 12 weeks of arsenic exposure, arsenic exposure group was divided into three groups: natural recovery group ( n=8), low-dose intervention group (n=8) and high-dose intervention group ( n=8). The rats were treated with 2-APB for 21 days. After the last administration, the routine parameters and the layered strain parameters were measured by ultrasonic instrument. Then the rats were killed and their blood and myocardial samples were obtained. The levels of serum creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) were tested, and the morphological changes of cardiomyocytes were observed by HE staining. Results:Left ventricular ejection fraction (LVEF), left ventricular short axis shortening (LVFS), global circumferential strain of subendocardial myocardium (GCS-endo), global circumferential strain of middle myocardium (GCS-mid), global circumferential strain of subepicardial myocardium (GCS-epi) and circumferential strain rate of left ventricular segments (SrC) in the natural recovery group were lower than those in the control group ( P<0.05), while serum CK-MB and LDH were higher than those in the control group ( P<0.05). Some ultrasonic parameters and biochemical indexes in the low and high dose intervention groups were improved in varying degrees compared with the natural recovery group ( P<0.05). The correlation between GCS-endo and CK-MB was the highest ( r=-0.931, P<0.05). Myocardial HE staining showed that the degree of myocardial cell swelling and necrosis were reduced, and erythrocyte exudation was reduced in the low and high dose intervention groups compared with the natural recovery group. Conclusions:The stratified strain technique can be used to evaluate the protective effect of 2-APB on cardiac dysfunction in arsenic poisoning rats, and GCS-endo may be one of its sensitive indexes.
