Lipoic acid suppresses compound 48/80-induced anaphylaxis-like reaction.
10.5115/acb.2010.43.4.317
- Author:
Yun Ho CHOI
1
;
Ok Hee CHAI
;
Eui Hyeog HAN
;
Su Young CHOI
;
Hyoung Tae KIM
;
Chang Ho SONG
Author Information
1. Department of Anatomy, Chonbuk National University Medical School, Institute for Medical Sciences, Chonbuk National University, Jeonju, Korea. asch@jbnu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Mast cells;
Immediate-type hypersensitivity;
Histamine;
Calcium;
cAMP
- MeSH:
Anaphylaxis;
Animals;
Atherosclerosis;
Calcium;
Diabetic Neuropathies;
Formaldehyde;
Histamine;
Histamine Release;
Inflammation;
Mast Cells;
Mice;
Plasma;
Rats;
Thioctic Acid;
Toluene
- From:Anatomy & Cell Biology
2010;43(4):317-324
- CountryRepublic of Korea
- Language:English
-
Abstract:
Alpha-lipoic acid (LA), a naturally occurring dithiol compound, is an essential cofactor in metabolic reactions involved in energy utilization. LA improves glycemic control, reduces diabetic polyneuropathies, atherosclerosis, and allergic inflammation. The effects of LA on mast cell-mediated anaphylactic reactions, however, are unknown. LA dose-dependently inhibited systemic and passive cutaneous anaphylaxis-like reactions in mice induced by compound 48/80, a condensation product of N-methyl-p-methoxyphenethylamine and formaldehyde. Pretreatment with LA, prior to induction of the systemic anaphylaxis-like reaction with compound 48/80, reduced plasma histamine levels in a dose-dependent manner. In our in vitro study, LA decreased histamine release from rat peritoneal mast cells (RPMCs) triggered by compound 48/80. Moreover, an increase in calcium uptake activated by compound 48/80 was inhibited by LA. LA also significantly elevated intracellular cyclic adenosine-3',5' monophosphate (cAMP) levels in RPMCs. This inhibition of mediator release from RPMCs may be due to inhibition of calcium uptake and augmentation of intracellular cAMP levels. Based on these results, we suggest that LA may be a potential remedy for allergy-related diseases.
