Expression and correlation analysis of H19, MMP-2, MMP-9 in patients with recurrent spontaneous abortion
10.3760/cma.j.cn431274-20210303-00254
- VernacularTitle:H19及MMP-2、MMP-9在复发性自然流产患者中的表达及相关性分析
- Author:
Lian HU
1
;
Lijun LI
;
Yan XU
;
Shaoping HUANG
;
Dan LIU
;
Jun ZHANG
Author Information
1. 长沙市第四医院妇产科 410006
- Keywords:
Abortion, habitual;
Matrix metalloproteinase 2;
Matrix metalloproteinase 9;
H19 gene
- From:
Journal of Chinese Physician
2021;23(12):1800-1804,1809
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and correlation of H19, matrix metalloproteinase (MMP)-2 and MMP-9 in patients with recurrent spontaneous abortion (RSA).Methods:Human extravillous trophoblast cell line HTR-8 was cultured in vitro. Lentivirus was used to infect the HTR-8 cell line to over-express or knockdown the expression of H19. The concentrations of MMP-2 and MMP-9 protein in cell culture supernatant were detected by enzyme linked immunosorbent assay (ELISA). The expression levels of H19, MMP-2 and MMP-9 mRNA in villi of patients with RSA were detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). Spearman correlation analysis was used to understand the correlation between H19 and the expression levels of MMP-2 and MMP-9. Results:After overexpression of H19, the expression levels of MMP-2 and MMP-9 mRNA and protein concentration in Lv-ph19 group were significantly higher than those in Lv-vector group ( P<0.05); After interfering with the expression of H19, the expression levels of MMP-2 and MMP-9 mRNA and protein concentration in Lv-shH19 group were significantly lower than those in Lv-shcon control group ( P<0.05). The number of spontaneous abortions in patients with recurrent spontaneous abortion was significantly higher than that in the control group ( P<0.05). qRT-PCR showed that the expression levels of H19, MMP-2 and MMP-9 mRNA in villi of patients with RSA were significantly lower than those in the control group ( P<0.05). There was a positive correlation between H19 and the expression levels of MMP-2 and MMP-9 ( P<0.05). Conclusions:H19 regulates the expression of MMP-2 and MMP-9 of trophoblast during early pregnancy, and the abnormal expression of H19, MMP-2, and MMP-9 in human first-trimester villous tissues was related with the incidence of early miscarriage.
